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Proteinase K, 100 mg, 1 x 100 mg

≥30 mAnson U/mg, lyophilized
Icon_DNAse-free
Icon_RNAse-free
Przykłady działania: prowadzą do uszczerbku na zdrowiu, drażnią oczy, skórę lub organy układu oddechowego. W dużych ilościach prowadzą do śmierci. Bezpieczeństwo: jak wyżej, w razie podrażnienia skóry lub kontaktu z oczami przepłukać wodą lub innym odpowiednim środkiem.
Przykłady działania: działają alergizująco, rakotwórczo, mutagennie, szkodliwie na rozrodczość lub na narządy Bezpieczeństwo: przed rozpoczęciem pracy z takimi substancjami należy się dobrze poinformować; nosić odzież ochronną i rękawice, ochronę oczu i ust lub ochronę dróg oddechowych.
Danger
H315-H317-H319-H334-H335
i causes skin irritation, may cause an allergic skin reaction, causes serious eye irritation, may cause allergy or asthma symptoms or breathing difficulties if inhaled, may cause respiratory irritation
P260 P280 P342+P311
i do not breathe dust, wear protective gloves/eye protection, if experiencing respiratory symptoms: Call a POISON CENTER/doctor
Pack Qty.
Pack.
Packaging
Storage temp. +4 °C
WGK 1
CAS No. [39450-01-6]
EG-Nr. 254-457-8

Non-specific protease for degrading proteins in biological samples. Isolated from fungi (Tritirachium album).
Art. No. 7528.3: A The stock solution (20 mg/ml) can be prepared directly in the bottle.

Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Szczegóły dotyczące produktu
Guarantee analysis

94,90 €/op. 

plus VAT | 100 mg za op.

Nr kat. 7528.1

Dostępny z magazynu w Karlsruhe
Szybka, prosta i niezawodna dostawa.
od 6 op. 90,16 €/op.
od 24 op. 85,41 €/op.
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Szczegóły dotyczące produktu


Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.


Directions for use

Activity: (Haemoglobin, pH 7.5; 25 °C) 30 mAnson-U/mg
Foreign activity: RNase and DNase not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.


Working concentration

Stock solution: 20 mg/ml in water
Storage temperature (stock solution): -20 °C
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS
Working solution: 50 µg/ml



Proteinase K ≥30 mAnson U/mg, lyophilized

A non-specific protease with strong proteolytic activity for degrading proteins in biological samples. A quality product for molecular biology with a broad scope of application.

Application examples:
  • Preparing chromosomal DNA for PFGE
  • Degrading nucleases during preparation of nucleic acids (includes preparation of native RNA)

Art. No. 7528.3: A stock solution with a concentration of 20 mg/ml can be prepared directly by adding 25 ml buffer or water.



Proteinase K
Wybrana ilość:   0
  1. Suma pośrednia:  0.00
Nr kat. Pack Qty. Pack. Packaging Cena Ilość
7528.1 100 mg glass 1 x 100 mg 94,90 €
7528.2 500 mg glass 1 x 500 mg 317,50 €
7528.3 500 mg glass in 30 ml glass vial 355,00 €
7528.4 1 g glass 1 x 1 g 529,00 €
7528.5 250 mg glass 1 x 250 mg 177,50 €
7528.6 5 g glass 1 x 5 g 2.119,00 €
Dostępny z magazynu w Karlsruhe
Dostępny krótkoterminowo
Niedostępny
Data dostawy obecnie nieznana
Wybrana ilość:   0
  1. Suma pośrednia:  0.00

Pobranie


Informacje ogólne

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

Oxidoreductases (catalyse redox reactions)

Transferases (transfer functional groups among substrates)

Hydrolases (cleave bonds via addition of water)

Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

Isomerases (transform chemical isomers)

Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


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Guarantee analysis

Activity (Hemoglobin, 37 °C)≥30 U/mg  
DNases, RNasesnot detected  
Endonucleasesnot detected  

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