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6-Carboxyfluorescein Azide (6-FAM-Azide), 1 mg

≥95 %
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Pack.
4-((3-Azidopropyl)carbamoyl)-2‑(6-hydroxy-3-oxo-3H-xanthen-9‑yl)benzoic acid, 6-FAM-Azide
Empirical formula C24H18N4O6
Molar mass (M) 458,43 g/mol
Storage temp. -20 °C
Transport temp. cooled
WGK 1

Fluorescent dye

Type analysis

45,90 €/op. 

plus VAT | 1 mg za op.

Nr kat. 7806.1

Dostępny z magazynu w Karlsruhe
Szybka, prosta i niezawodna dostawa.
od 6 op. 43,60 €/op.
od 24 op. 41,31 €/op.
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Informacje techniczne
Emissions maximum 516 nm  
6-Carboxyfluorescein Azide (6-FAM-Azide)
Wybrana ilość:   0
  1. Suma pośrednia:  0.00
Nr kat. Pack Qty. Pack. Cena Ilość
7806.1 1 mg plastic 45,90 €
7806.2 5 mg plastic 78,90 €
7806.3 10 mg plastic 117,50 €
7806.4 100 mg plastic 459,00 €
Dostępny z magazynu w Karlsruhe
Dostępny krótkoterminowo
Niedostępny
Data dostawy obecnie nieznana
Wybrana ilość:   0
  1. Suma pośrednia:  0.00

Pobranie


Informacje ogólne

Click Chemistry

The copper(I)‑catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.


References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.


Tags

Fluorescent Tags
Carl ROTH offers a selection of fluorescent tags for labelling of (bio-)molecules via click chemistry. All available tags are suitable for fluorescence spectroscopy applications due to their excellent absorption properties and good solubility. Tags with PEG-spacer are beneficial for applications with as little interaction between the analyte and the tag as possible. The coumarine derivative (Art No. 7811) offers a unique feature: fluorescence is only detected after the coupling via click reaction.


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Type analysis

Appearanceorange solid  
Assay (HPLC)≥95 %  
Absorption max. (buffer, pH 9)496 nm  
Emission max. (buffer, pH 9)516 nm  
Molar extinction coefficient ε83 000 cm -1M-1  
SolubilityDMSO, DMF, MeOH  

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