Przejdź do treści Przejdź do menu nawigacyjnego

ROTI®Sep 1077 human, 100 ml

CELLPURE® sterile, ready-to-use, for density gradient centrifugation
Icon_Steril Sterile products
Icon_ready-to-use Ready-to-use products and reagents
Przykłady działania: działają alergizująco, rakotwórczo, mutagennie, szkodliwie na rozrodczość lub na narządy Bezpieczeństwo: przed rozpoczęciem pracy z takimi substancjami należy się dobrze poinformować; nosić odzież ochronną i rękawice, ochronę oczu i ust lub ochronę dróg oddechowych.
Danger
H317-H334
i may cause an allergic skin reaction, may cause allergy or asthma symptoms or breathing difficulties if inhaled
P302+P352 P333+P313
i IF ON SKIN: Wash with plenty of water, if skin irritation or rash occurs: Get medical advice/attention
Pack Qty.
Pack.
Lymphocyte separation medium, Polysucrose solution
Molar mass (M) ~400000 g/mol
Density (D) 1,08 g/cm³
Boiling point (bp) 100 °C
Storage temp. +4 °C
WGK 3

Polysucrose based medium for separation of lymphocytes/PBMC from human whole blood. Density: 1,077 g/ml
Long-term storage at approx. 4 °C recommended. Short-term storage at room temperature possible. Storage protected from light.

Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymp ...
Szczegóły dotyczące produktu
Type analysis
42,50 € /op.

32,90 €/op.  Campaign price!

plus VAT | 100 ml za op.

Nr kat. 0642.1

Dostępny z magazynu w Karlsruhe
Szybka, prosta i niezawodna dostawa.
Usługa abonamentowa firmy Carl Roth

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:
1

Put all products for your subscription in the desired quantity in the basket.

2

In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.

3

Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Szczegóły dotyczące produktu


Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation.
Density 1.077 g/ml.
Best alternative to Ficoll®.

  • Optimised for isolation of lymphocytes from non-coagulated human whole blood
  • Produces distinct, compact layers
  • Results in high recovery rates of viable cells
  • Maintains the representative ratio of B- to T lymphocytes
  • May be directly applied to all protocols using Ficoll®

ROTI®Sep 1077 human excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ratio of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range.

Suitable for application with human non-coagulated peripheral blood. In all respective protocols originally using Ficoll® this reagent may be replaced by ROTI®Sep 1077 human without any adjustment.



ROTI®Sep 1077 human CELLPURE® sterile, ready-to-use, for density gradient centrifugation

For research use only. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro diagnostics.

0642_02
The figure depicts two specimens prepared with heparinic blood obtained from venous puncture. Density gradient centrifugation of 6 ml diluted whole blood (dilution in PBS 1:1.5), with 3 ml of ROTI®Sep 1077 human, 10 mins., 1000 g, no break.


ROTI®Sep 1077 human
Wybrana ilość:   0
  1. Suma pośrednia:  0.00
Nr kat. Pack Qty. Pack. Cena Ilość
0642.1 100 ml plastic 32,90 €
0642.2 500 ml plastic 88,80 €
Dostępny z magazynu w Karlsruhe
Dostępny krótkoterminowo
Niedostępny
Data dostawy obecnie nieznana
Wybrana ilość:   0
  1. Suma pośrednia:  0.00

Pobranie

Świadectwa jakości

Tu możesz wyszukać i pobrać świadectwo jakości wybranego produktu. Wpisz numer serii.
Znaleziono następujące świadectwa jakości:

Type analysis

Appearance of solutionclear, colourless  
Density1,077 ±0,001 g/ml  
pH value7,0 ±0,5  
Osmolality325 ±35 mOsm/kg  
Sterilitycomplies  
Endotoxinstested  
For research use only. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro diagnostics.

0 Oceny

0.0 z 5 gwiazdek