Technical Data Sheet
ClickTech DNA FISH Kit 594
All products identified as being particularly temperaturesensitive are shipped in special ice boxes with freezer packs or in dry ice.
Ready-to-use products and reagents
Transport temp. cooled
WGK 1
Preparation of fluorescent-labeled DNA probes by PCR
€709.50/Pack Qty.
excl. VAT. | 1 kit per Pack Qty.
Art. No. 1Y7T.1
Product details
FISH (fluorescence in-situ hybridisation) is a molecular cytogenetic technique that enables the detection and localisation of specific DNA sequences in cells or tissues. It is often used in clinical applications such as cancer diagnosis and prognosis.
ClickTech DNA FISH Kit 594 for Fluorescence in situ hybridization (FISH)
- dNTP Mix (10 mM)
- EdUTP (5 mM)
- baseclick Ethynyl Polymerase (2 U/µl)
- PCR Buffer (5x)
- 10x Activator2
- 5/6-Sulforhodamine-101-PEG3-Azide
- Reactor 25
| λ | 594 nm |
| Markers | 5/6-Sulforhodamine-101-PEG3-Azide |
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| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 1Y7T.1 | 1 kit | cardboard | For performing 20 PCR reactions (50 µl each) and 40 independent labeling reactions (FISH experiments). |
€709.50 |
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Delivery date currently unknown
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Downloads / MSDS
General information
The copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.
References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.
In recent years, click chemistry has become increasingly important and steadily more popular in research and development. The reasons for this are not only the easy handling and the high yields but also the countless application possibilities. Carl ROTH offers you the possibility to purchase the broad portfolio of click reagents, various kits for EdU-based cell proliferation assays and numerous other applications from the well-known brand baseclick. This enables us to offer you a necessary and comprehensive assortment for click chemistry.