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Technical Data Sheet
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Blotting-Sandwich ROTI® ProBlot PVDF 0.2
From  Carl ROTH
Icon_ready-to-use Ready-to-use products and reagents
Material: polyvinylidene fluoride (PVDF).
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Blotting Sandwich – Simple & Efficient

Our blotting sandwiches offer a time-saving and reliable solution for the efficient transfer of proteins and nucleic acids. They consist of perfectly matched, pre-cut components, including our high-quality ROTI®ProBind membranes, which ensure excellent binding capacity and minimal background interference.
The easy handling reduces sources of error, optimizes reproducibility, and simplifies the workflow. This allows you to achieve consistent and precise results—without any additional preparation effort. Ideal for any laboratory!
Product details

€387.00/Pack Qty. 

excl. VAT. | 20 unit(s) per Pack Qty.

Art. No. 3A88.1

In stock
Delivery fast, simple and reliable!
from 6 Pack Qty. €367.65/Pack Qty.
from 24 Pack Qty. €348.30/Pack Qty.

Product details

Material: polyvinylidene fluoride (PVDF).

Blotting Sandwich – Simple & Efficient

Our blotting sandwiches offer a time-saving and reliable solution for the efficient transfer of proteins and nucleic acids. They consist of perfectly matched, pre-cut components, including our high-quality ROTI®ProBind membranes, which ensure excellent binding capacity and minimal background interference.
The easy handling reduces sources of error, optimizes reproducibility, and simplifies the workflow. This allows you to achieve consistent and precise results—without any additional preparation effort. Ideal for any laboratory!



Blotting-Sandwich ROTI® ProBlot PVDF 0.2 

Applications:
  • Western Blotting
  • Southern Blotting
  • Northern Blotting
  • Amino Acid and Protein analysis


Not a medical device / Not an IVD product
Technical Information
Pore size 0,2 µm
Bond hydrophobic 
Thickn. 180±20 µm
Immobilisation not necessary 
Material polyvinylidene fluoride 
Binding capacity ≥260 µg/cm²
Blotting-Sandwich ROTI® ProBlot PVDF 0.2
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Art. No. Pack Qty. Price Quantity
3A88.1 20 unit(s) 

€387.00

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  1. Subtotal:  0.00

Downloads / MSDS

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General information

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Transfer membrane ROTI®PVDF 0.2 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,2 not necessary
Transfer membrane ROTI®PVDF 0.45 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Transfer membrane ROTI®NC 0.2 Nitrocellulose hydrophobic 150 ±50 100 0,2 UV, baking (max. 80 °C)
Transfer membrane ROTI®NC 0.45 Nitrocellulose hydrophobic 150 ±50 100 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not