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Transfer membrane ROTI® ProBind NC 0.2, 300 × 30 cm
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WGK 1
UN-Nr. 3270
ProBind transfer membranes are the ideal choice for reliable protein analysis, Southern and Northern blots. Available as PVDF or nitrocellulose (NC) membranes, they offer high mechanical stability, excellent binding capacity, and minimal background interference. Their uniform structure ensures sharp and clear bands for precise results.Thanks to their high chemical resistance and optimal sensitivity, ProBind transfer membranes are perfect for a wide range of detection methods—from classic blotting techniques to advanced assays.
516,00 €/VE
Excl. btw | 1 rol(len) Per VE
Bestelnr. 3A7E.1
Productdetails
Transfer Membrane for Nucleic Acid and Protein Immobilization Techniques
ProBind transfer membranes are the ideal choice for reliable protein analysis, Southern and Northern blots. Available as PVDF or nitrocellulose (NC) membranes, they offer high mechanical stability, excellent binding capacity, and minimal background interference. Their uniform structure ensures sharp and clear bands for precise results.Thanks to their high chemical resistance and optimal sensitivity, ProBind transfer membranes are perfect for a wide range of detection methods—from classic blotting techniques to advanced assays.
Transfer membrane ROTI® ProBind NC 0.2
- Western Blotting
- Southern Blotting
- Northern Blotting
- Amino Acid and Protein Analysis
| Afmeting | 300 × 30 cm |
| Uitvoering | Roll |
| Poriëngr. | 0,2 µm |
| Binding | hydrophobic |
| Dikte | 145±20 µm |
| Immobilisering | not necessary |
| Bindingscapaciteit | ≥160 µg/cm² |
- Tussentotaal: 0.00
| Bestelnr. | Afmeting | VE | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|
| 3A7C.1 | 10 × 10 cm | 25 blad |
268,75 € |
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| 3A7E.1 | 300 × 30 cm | 1 rol(len) |
516,00 € |
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Op voorraad
Beschikbaar (let op levertijd)
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
Technical Parameters of ROTH-Transfer Membranes
| Membrane | Material | Characteristics | Thickness (μm) | Binding capacity (μg/cm2) | Pore size (μm) | Immobilisation |
| Roti® Fluoro PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | 300 | 0,2 | not necessary |
| Transfer membrane ROTI®PVDF 0.2 | Polyvinyliden fluoride | hydrophobic | 190 ±50 | ≥125 | 0,2 | not necessary |
| Transfer membrane ROTI®PVDF 0.45 | Polyvinyliden fluoride | hydrophobic | 190 ±50 | ≥125 | 0,45 | not necessary |
| Immobilon P | Polyvinyliden fluoride | hydrophobic | 120 ±25 | 150-300 | 0,45 | not necessary |
| Roti® Nylon 0.2 | Nylon 6,6 | neutral/amphoteric | 150 ±20 | 500 | 0,2/0,45 | UV, baking |
| Roti® Nylon plus | Nylon 6,6 | hydrophilic, positively charged | 160 ±20 | 500 | 0,45 | not necessary, UV and baking possible |
| Transfer membrane ROTI®NC 0.2 | Nitrocellulose | hydrophobic | 150 ±50 | 100 | 0,2 | UV, baking (max. 80 °C) |
| Transfer membrane ROTI®NC 0.45 | Nitrocellulose | hydrophobic | 150 ±50 | 100 | 0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® | Nitrocellulose | electrostatic, hydrophobic | unknown | 11-72 | 0,2/0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® supported | Nitrocellulose | electrostatic, hydrophobic | unknown | 86-110 | 0,2/0,45 | UV, baking (max. 80 °C) |
| Biomolecules / Membrane Pores | Size (kD) |
| genomic DNA (human) | >11000000 |
| E. coli | approx. 1500000 |
| 0.45 μm | approx. 5000 |
| 0.2 μm | approx. 2000 |
| DNA 3 kb | approx. 1000 |
| RNA 1 kb | approx. 300 |
| Antibodies (IgG) | approx. 150 |
| Proteins in the lab | approx. 10–300 |
| BSA (Albumin) | 69 |
| Biomolecules | Nylon plus | Nylon 0.2 | PVDF 0.2 | PVDF | Fluoro PVDF | NC | NC 0.45 |
| DNA | +++ | + | - | - | - | + | + |
| RNA | + | +++ | - | - | - | + | + |
| Proteins | - | + | +++ | +++ | +++ | + | + |
| Detection System | |||||||
| Colour | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Radioactivity | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Chemoluminescence | +++ | +++ | +++ | +++ | - | + | + |
| Fluorescence | +++ | +++ | - | - | +++ | - | - |
| +++: recommended | +: suitable | -: not | |||||