Technical Data Sheet
anti-Keratin Type II mouse monoclonal, Ks pan1-8, 0.2 ml
Bitte beachten: Abweichende Konditionen / Please note: Different conditions
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.
€101.50/Pack Qty.
excl. VAT. | 0.2 ml per Pack Qty.
Art. No. 1NNH.1
Alternative products
Fits to / Accessories
Product details
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-Keratin Type II mouse monoclonal, Ks pan1-8 50 μg/ml, monoclonal, affinity purified
Ks pan1-8 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors.
Polypeptides reacting: Mr 52,500-Mr 68,000 keratins (type II keratins K1-K8; formerly also designated cytokeratins 1-8) of human epithelial cells.
Tumors specifically detected: all epithelium-derived neoplasms.
Reactivity on cultured cell lines MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12.
Immunocytochemistry (ICC): Assay dependent
Immunohistochemistry (IHC) - frozen: 1:10-1:100 (0.5-5 μg/ml)
Immunohistochemistry (IHC) - paraffin: 1:10-1:100 (0.5-5 μg/ml, microwave treatment recommended)
Western Blot (WB): 1:500 (0.1 μg/ml)
| Application |
ICC/IF IHC WB |
| Host species | mouse |
| Clonality | monoclonal |
| Reactivity antibody |
mouse human rat cattle Amphibians |
| Purification | Affinity chromatography |
| Conjugate | uncojugated |
| Concentration | 50 µg/ml |
| Isotype | IgG2a |
| Clone | Ks pan1-8 |
| Target | Cytoskeletal proteins from cultured human MCF-7 cells |
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| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 1NNH.1 | 0.2 ml | plastic |
€101.50 |
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| 1NNH.2 | 1 ml | plastic |
€265.00 |
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- Subtotal: 0.00
Downloads / MSDS
General information
Secondary antibodies do not bind directly to the target molecule, but rather to the primary antibody. They are usually coupled with enzymes or fluorescent dyes so that the binding of the primary antibody can be made visible or measurable. In research, they serve as signal amplifiers and enable more sensitive detection, as several secondary antibodies can bind to one primary antibody. This makes them important tools in methods such as ELISA, Western blot or microscopy.
Primary antibodies are proteins that bind specifically to a particular antigen, i.e. a target molecule such as a protein or a structure within a cell. They are used in research to visualise specific molecules, isolate them or investigate their function. Typical applications include Western blotting, immunohistochemistry and flow cytometry. Due to their high specificity, primary antibodies enable the precise detection of biological processes.