Signal Enhancer Western/ELISA Set, 2 x 50 ml
All products identified as being particularly temperaturesensitive are shipped in special ice boxes with freezer packs or in dry ice.
Transport temp. cooled
One set with 250 ml each is sufficient for optimization of approx. 25 minigel blots.
€144.60/Pack Qty.
excl. VAT. | 1 kit per Pack Qty.
Art. No. 9211.2
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Product details
Kit for amplification of immunochemical detection signals on blot membranes and in ELISA.
- Strong enhancement of signal strength
- Most easy application
The Signal Enhancer Western/ELISA enhances antigen-antibody reactions in a variety of immunoassays such as Western blotting, dot blotting and ELISA. It can significantly enhance detection of weak immunoreactive and low abundance target proteins and is well compatible with all common assay versions, membranes and ELISA plates.
Signal enhancement is dependent on several parameters such as the particular antigen/antibody pair, the particular detection enzyme, the substrate chosen and so on. In most cases, however, the signal strength is about 5 to 10fold higher than with the substrate alone.
- Western and ELISA
- HRP and AP
- all common membranes
- all common substrates
For application, the two solutions Signal Enhancer Western/ELISA-1 and -2 are simply used as buffer solutions for preparation of the primary- and secondary antibody incubation solutions. Following incubation and washing, all common detection procedures may be applied, for instance colour staining with ROTI®DAB-Kit (Art. No. 9202) or chemoluminescent staining with ROTI®Lumin plus (Art. No. 3692).
Signal Enhancer Western/ELISA Set for immunochemistry
A: Base method w/o enhancer, B: Detection via Signal Enhancer Western/ELISA
With kind permission of Dr. K. Ishizuka, Department of Psychiatry and Behavioural Sciences, Johns Hopkins University, Baltimore, USA
Signal Enhancer Western/ELISA-1 (Art. No. 9213) and Signal Enhancer Western/ELISA-2 (Art. No. 9214). 1 Mini-Set: 2 x 50 ml, 1 Set: 2 x 250 ml.
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| Order No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
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| 9211.1 | 1 kit | plastic | 2 x 250 ml | €424.65 |
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| 9211.2 | 1 kit | plastic | 2 x 50 ml | €144.60 |
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- Subtotal: 0.00
Downloads
Certificates of Analysis
Type analysis
| Application test (ELISA) | complies |