Technical Data Sheet
anti-Cardiac Actin mouse monoclonal, AC1-20.4.2, 1 ml
Bitte beachten: Abweichende Konditionen / Please note: Different conditions
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.
€284.90/Pack Qty.
excl. VAT. | 1 ml per Pack Qty.
Art. No. 1NLN.2
Alternative products
Fits to / Accessories
Product details
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-Cardiac Actin mouse monoclonal, AC1-20.4.2 50 μg/ml, monoclonal, affinity purified
Ac1 represents an excellent marker for cardiac tissue; it discriminates fetal (cardiac) a-actin from all other actin isoforms. Fetal actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord. Mab Ac1-20.4.2 shows no cross reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied.
Polypeptide reacting: specific for fetal (cardiac) isoform of actin.
Immunohistochemistry (IHC) - frozen: 1:10 (5 μg/ml; include 0.5 M NaCl in all washing buffers to enhance specificity)
Immunohistochemistry (IHC) - paraffin: 1:10 (5 μg/ml; microwave treatment recommended)
Western Blot (WB): 1:1,000 (50 ng/ml; include 1 M NaCl in all washing buffers to enhance specificity)
| Application |
IHC WB |
| Host species | mouse |
| Clonality | monoclonal |
| Reactivity antibody |
cattle chicken human mouse rabbit |
| Purification | Affinity chromatography |
| Conjugate | uncojugated |
| Concentration | 50 µg/ml |
| Isotype | IgG1 |
| Uniprot ID AK | P68034 (chicken), P68032 (human), G1STB6 (rabbit) |
| Clone | AC1-20.4.2 |
| Target | cardiac a-actin |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 1NLN.1 | 0.2 ml | plastic |
€109.15 |
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| 1NLN.2 | 1 ml | plastic |
€284.90 |
|
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In stock
Available (note delivery time)
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No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
Secondary antibodies do not bind directly to the target molecule, but rather to the primary antibody. They are usually coupled with enzymes or fluorescent dyes so that the binding of the primary antibody can be made visible or measurable. In research, they serve as signal amplifiers and enable more sensitive detection, as several secondary antibodies can bind to one primary antibody. This makes them important tools in methods such as ELISA, Western blot or microscopy.
Primary antibodies are proteins that bind specifically to a particular antigen, i.e. a target molecule such as a protein or a structure within a cell. They are used in research to visualise specific molecules, isolate them or investigate their function. Typical applications include Western blotting, immunohistochemistry and flow cytometry. Due to their high specificity, primary antibodies enable the precise detection of biological processes.
Certificates of Analysis
Type analysis
| Clonality | Monoclonal |
| Concentration | 50 µg/ml |
| Raised in | Hybridoma cell culture supernatant |
| Immunglobulin (Ig) class | IgG1 |
| Clone | AC1-20.4.2 |
| Conjugate | Unconjugated |
| Purification | Affinity chromatography |
| Purity (SDS-PAGE) | ≥95 % |
| PBS buffer, pH 7.4 with 0.09 % sodium azide and 0.5 % BSA., Short term at 2-8 °C (+4 °C); long term storage in aliquots at -20 °C; avoid freeze/thaw cycles. | |