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anti-p53 Protein mouse monoclonal, Bp53.11, 1 ml

From  PROGEN
50 μg/ml, monoclonal, affinity purified
Icon_Abweichende-Konditionen Bitte beachten: Abweichende Konditionen / Please note: Different conditions
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Pack Qty.
Pack.
Antibody, Primary antibodies
Density (D) ~1 g/cm³
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.

Cell cycle marker

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
Product details

€274.15/Pack Qty. 

excl. VAT. | 1 ml per Pack Qty.

Art. No. 1NNY.2

Available (note delivery time)
Ready for dispatch in 1-2 weeks
Free of shipping costs from 250 €
from 6 Pack Qty. €260.44/Pack Qty.
from 24 Pack Qty. €246.74/Pack Qty.

Product details


This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

  • High epitope affinity
  • Highly specific detection of the target protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence


anti-p53 Protein mouse monoclonal, Bp53.11 50 μg/ml, monoclonal, affinity purified

Excellent marker for wild-type and mutant forms of human p53 antigen.

Epitope recognized by Bp53.11: 20-SDLWKLLPENNV-31.

The antibody stains positively approx. 60% of investigated carcinoma of lung, breast, colon, stomach, esophagus, pancreas, urinary bladder and testis, head and neck tumors; T-cell Leukemia, non-Hodgkin-Lymphoma, melanoma, sarcoma.

Reactivity on cultured cell lines: Hela, MCF-7.


Application

Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:1,000-1:3,000 (17-50 ng/ml)
Immunohistochemistry (IHC) - paraffin: 1:1,000-1:3,000 (17-50 ng/ml, microwave treatment recommended)
Western Blot (WB): 1:2,000-1:10,000 (5-25 ng/ml)


1NNY_01
p53 staining in human squamous cell carcinoma (image courtesy of J. Heß, University Hospital Heidelberg)
1NNY_04
p53 staining in human squamous cell carcinoma (image courtesy of J. Heß, University Hospital Heidelberg)
1NNY_06
Western blot analysis of HEK lysate with anti-p53 antibody. Western blot analysis was performed on 20 μg HEK lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-p53, mouse monoclonal, Bp53.11 (Cat. No. 690039) was diluted in blocking buffer (antibody concentration 25 ng/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.


Not a medical device / Not an IVD product
Technical Information
Application ICC/IF 
IHC 
WB 
Host species mouse 
Clonality monoclonal 
Reactivity antibody human 
Purification Affinity chromatography 
Conjugate uncojugated 
Concentration 50 µg/ml 
Isotype IgG2a 
Uniprot ID AK Q96A56 (human) 
Clone Bp53.11 
Target Recombinant human p53 (transcription domain within the NH2 terminus) 
anti-p53 Protein mouse monoclonal, Bp53.11
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Art. No. Pack Qty. Pack. Price Quantity
1NNY.1 0.2 ml plastic

€107.00

1NNY.2 1 ml plastic

€274.15

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In procurement
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Downloads / MSDS

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