Technical Data Sheet
anti-p53 Protein mouse monoclonal, Bp53.11, 1 ml
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.
€274.15/Pack Qty.
excl. VAT. | 1 ml per Pack Qty.
Art. No. 1NNY.2
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Fits to / Accessories
Product details
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-p53 Protein mouse monoclonal, Bp53.11 50 μg/ml, monoclonal, affinity purified
Excellent marker for wild-type and mutant forms of human p53 antigen.
Epitope recognized by Bp53.11: 20-SDLWKLLPENNV-31.
The antibody stains positively approx. 60% of investigated carcinoma of lung, breast, colon, stomach, esophagus, pancreas, urinary bladder and testis, head and neck tumors; T-cell Leukemia, non-Hodgkin-Lymphoma, melanoma, sarcoma.
Reactivity on cultured cell lines: Hela, MCF-7.
Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:1,000-1:3,000 (17-50 ng/ml)
Immunohistochemistry (IHC) - paraffin: 1:1,000-1:3,000 (17-50 ng/ml, microwave treatment recommended)
Western Blot (WB): 1:2,000-1:10,000 (5-25 ng/ml)
| Application |
ICC/IF IHC WB |
| Host species | mouse |
| Clonality | monoclonal |
| Reactivity antibody | human |
| Purification | Affinity chromatography |
| Conjugate | uncojugated |
| Concentration | 50 µg/ml |
| Isotype | IgG2a |
| Uniprot ID AK | Q96A56 (human) |
| Clone | Bp53.11 |
| Target | Recombinant human p53 (transcription domain within the NH2 terminus) |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 1NNY.1 | 0.2 ml | plastic |
€107.00 |
|
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| 1NNY.2 | 1 ml | plastic |
€274.15 |
|
|
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