Ethanol, 2.5 l, glass

Molisch’s test:

Molisch’s reagent is added to a diluted sample of monosaccharide and concentrated sulphuric acid is added down the sides of the test tube to form a layer beneath the solution. A purple ring forms at the interface between the layers. Since nucleic acids and glycoproteins also contain monosaccharides, they may react as well.

Selivanov’s test:

Selivanov’s test involves acidifying a sugar solution with concentrated hydrochloric acid, adding an ethanolic resorcinol solution and heating. Ketoses react quickly to form a red-coloured dye, while aldoses do not react at all, or only react slowly.

Highest purity and extensive trace analysis for excellent results!

We offer:ROTIPURAN^® p.a. solvents with superior analytical reliability in terms of quality and batch consistency. The so branded solvents arebeing constantly controlled, guaranteed, and safeguarded through careful preparation, testing and packaging. In addition, many of these solvents meet the requirements of ACS (American Chemical Society) and ISO (International Organization for Standardization), and thusare fully specified.

Applications:ROTIPURAN^® p.a. solvents are suitable for all standard applications in biological and chemical laboratory or pilot plant, such as:extractions, qualitative and quantitative analysis. As a matter of course, the p.a. solvents offered here may well be used for otherapplications instead.

Carl ROTH offers an extensive range of calibration and control standards. These products are manufactured from highly pure starting material and are specially tested for each application. The corresponding batch-specific certificate of analysis is available online. Each product series offers excellent value for money.

Determination of Fat

Fat analysis is used to determine the fat content and fat composition of foodstuffs. Individual components or total fats levels can be calculated depending on the purpose of testing. Different components are subject to different procedures by law, such as the Gerber method or the Van Gulik technique.

• Add sample to 60 ml water

• Subsequently add, while mixing after each step:

  a) 5 ml Carrez-I-solution, Art. No. 9944

  (or 7,2 g zinc sulphate heptahydrate, Art. No. K301 ad 100 ml)

  b) 5 ml Carrez-II-solution, Art. No. 9950

  (or 3,6 g potassium hexacyanoferrate(II), Art. No. P745 ad 100 ml)

• Adjust pH to 7,5 - 8,5 by adding NaOH (0,1 M) Art.No. K020

• Fill volumetric flask up to mark, mix, and filter