Technical Data Sheet
Methylene blue (C. I. 52015), 100 g, glass
Molar mass (M) 319,9 g/mol
Melting point (mp) 190 °C
WGK 1
CAS No. 61-73-4
EG-Nr. 200-515-2
For microscopy and staining of nucleic acids in polyacrylamide gels.
Application note:
Staining of nucleic acids in PAA
Nucleic acids are visible as blue bands.
Detection limit: 20 ng/band.
€109.00/Pack Qty.
excl. VAT. | 100 g per Pack Qty.
Art. No. A514.3
Product details
Methylene blue (C. I. 52015) pure
Watersoluble nuclear dye, does not intercalate between base pair stacks of DNA. Especially used for staining of blood cells.
Application note:
Staining of nucleic acids in PAA
Nucleic acids are visible as blue bands.
Detection limit: 20 ng/band.
- Use a 0.2 % solution of methylene blue for staining
- Stain for 20 min.
- Wash for 15 min. in distilled water
| Dyeing | dead cells blue |
| Ox. colour | blue |
| Red. colour | colourless |
| Redox potential E0[V] at pH=0 and 20 °C | +0.53 (30 °C) |
| Use | vitality test |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| A514.1 | 10 g | glass |
€24.50 |
|
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| A514.2 | 50 g | glass |
€64.50 |
|
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| A514.3 | 100 g | glass |
€109.00 |
|
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| A514.4 | 250 g | glass |
€215.00 |
|
|
| A514.5 | 500 g | glass |
€389.00 |
|
|
| A514.6 | 1 kg | glass |
€619.00 |
|
|
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In stock
Available (note delivery time)
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
For brilliant results in the laboratory and research
Discover our extensive range of dyes for demanding applications in science and industry. Whether for microscopy, histology, biochemistry or industrial applications, you will find the right dye here.
The content of colourants is not specified for many products for various reasons:
1. complex composition: colourants are often mixtures of several components that may be present in different ratios. These compositions can vary, which makes it difficult to state an exact content.
2. application focus: In many applications, especially in the textile and food industries, the exact concentration of a dye is less relevant than its colouring power or its ability to achieve a certain colour. Here, the colour strength or the ability to impart colour is the most important criterion.
3. standardisation: Many dyes are traded according to certain standards (e.g. C.I. numbers) which guarantee a certain quality and colouring. These standards often make an exact specification of content superfluous, as users know that the colourant has the desired properties.
4. manufacturing processes: The manufacturing process of colourants can lead to natural variations in content. Instead of specifying an exact content, quality control is often carried out to ensure that the colourant is within an acceptable range.
5. Regulatory aspects: In some cases, regulatory requirements may not require the manufacturer to declare the exact content as long as the colourant is safe and effective for the intended purpose.
6. cost reasons: An exact determination of the content often requires complex analytical procedures that could increase production costs. Therefore, some manufacturers do not provide this additional information in order to save costs.
In summary, it can be said that the content of colourants is often not specified because other quality criteria are in the foreground and the exact concentration is not decisive for many applications.
Determination of Surfactants
Benzethonium chloride solution is used to measure anionic detergents. In this method, the anionic detergent and the cationic detergent form a 1:1 salt in dichloromethane. The end point of the titration is indicated by methylene blue.
Certificates of Analysis
Type analysis
| Appearance | Dark green crystalline powder |
| Assay (dry substance) | ≥95 % |
| Maximum absorption | 655-670 nm |
| Loss on drying (1 h, 100 °C) | ≤10 % |