Technical Data Sheet
Magnesium chloride solution, 1 ml, 2 x 0,5 ml in tubes
From Carl ROTH
25 mM, sterile, for molecular biology
Sterile
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Empirical formula MgCl2
Molar mass (M) 95,22 g/mol
Density (D) 1,003 g/cm³
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.
WGK 1
Molar mass (M) 95,22 g/mol
Density (D) 1,003 g/cm³
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.
WGK 1
For optimisation of PCR reaction conditions.
In a PCR reaction, cofactors such as divalent cations (MgCl2) are needed for an optimal process. Magnesium interacts with the DNA template, dNTPs and the polymerase in a PCR reaction. The MgCl2 concentration influences the productivity and accuracy of the polymerase. It also influences the binding of the primers to the template.
€12.90/Pack Qty.
excl. VAT. | 1 ml per Pack Qty.
Art. No. 1HY7.1
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from 6 Pack Qty.
€12.26/Pack Qty.
from 24 Pack Qty.
€11.61/Pack Qty.
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Product details
Sterile
Magnesium chloride solution 25 mM, sterile, for molecular biology
In a PCR reaction, cofactors such as divalent cations (MgCl2) are needed for an optimal process. Magnesium interacts with the DNA template, dNTPs and the polymerase in a PCR reaction. The MgCl2 concentration influences the productivity and accuracy of the polymerase. It also influences the binding of the primers to the template.
Application:
- Too high magnesium concentration can lead to reduced specificity and undesired PCR products
- Too low a magnesium concentration can lead to no PCR product at all
- Magnesium influences the melting point of double-stranded DNA
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| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 1HY7.1 | 1 ml | plastic | 2 x 0,5 ml in tubes |
€12.90 |
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| 1HY7.2 | 2.5 ml | plastic | 5 x 0,5 ml in tubes |
€22.90 |
|
|
|
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