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Gel Filtration Media ROTI®Dex-25 Medium, 50 g

Producent Carl ROTH
ROTI®Dex
Op.
Opak.
Dextran-Epichlorhydrin-Copolymer, Gel permeation chromatography (GPC), Size-exclusion chromatography (SEC)
Storage temp. +2 to +25 °C
WGK 1
CAS No. 9041-35-4

Suitable for proteins >5 kDa and oligonucleotides >10 bp.
For desalting and buffer exchange of protein and nucleic acid solutions.

ROTI®Dex Medium is a spherical, porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin.
Szczegóły dotyczące produktu

199,00 €/op. 

plus VAT | 50 g za op.

Nr kat. 21A5.2

Dostępny z magazynu w Karlsruhe
Szybka, prosta i niezawodna dostawa.
od 6 op. 189,05 €/op.
od 24 op. 179,10 €/op.

Szczegóły dotyczące produktu


ROTI®Dex Medium is a spherical, porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin.


In gel filtration, also called size exclusion chromatography, molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules.
In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins.


ROTI®Dex enables a form of group separation for the separation of undesired, low-molecular-weight factors such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.

Zalety:
  • Sample preparation in just a few minutes
  • Desalting, removal of impurities and buffer exchange in a single step
  • No interaction between analyte and medium
  • High selectivity, high resolution and high yield (> 95%)
  • Also suitable for small sample volumes
  • High chemical stability and therefore compatible with many organic and other solvents

Gel Filtration Media ROTI®Dex is supplied as a dry powder and must be swollen in an aqueous solution before use.
An efficient column packing is essential for a clean separation of biomolecules. Please refer to our recommended protocol in the instructions for use.

Gel Filtration Media ROTI®Dex-25 Medium ROTI®Dex

ROTI®Dex-25 medium is excellent for the separation of proteins >5 kDa and nucleic acids >10 bp. Biomolecules are not significantly diluted during purification with ROTI®Dex-25 medium.


Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.



Informacje techniczne
Matryca Cross-linked dextran 
MWCO 5 kDa
10 bp
Wielkość cząsteczek Dry: 50-150 µm
Wet: 85-260 µm
Gel Filtration Media ROTI®Dex-25 Medium
Wybrana ilość:   0
  1. Suma pośrednia:  0.00
Nr kat. Op. Opak. Cena Ilość
21A5.1 10 g plastic

81,50 €

21A5.2 50 g plastic

199,00 €

21A5.3 100 g plastic

349,00 €

21A5.4 250 g plastic

819,00 €

21A5.5 500 g plastic

1.495,00 €

Dostępny z magazynu w Karlsruhe
Dostępne (uwaga na czas dostawy)
Dostępny w późniejszym terminie
Niedostępny
Data dostawy obecnie nieznana
Wybrana ilość:   0
  1. Suma pośrednia:  0.00

Pobieranie plików / SDS


Informacje ogólne

Dalsze atrakcyjne oferty na ukończenie laboratorium chromatograficznego znajdziecie Państwo na naszej stronie Chromatografia!


The ROTI®Dex ready-to-use columns at a glance

Application Order No. Matrix Particle size (wet) Gel bed volume Sample volume MWCO Pack Qty.
Spin 21C4.1 ROTI®Dex 25 Medium 85-260 µm 0.5 ml 2-100 µl (50 µl optimal) Proteins >5 kDa,
Oligonucleotides >10 bp,
Nanoparticles >2 nm
25 Pcs
21C5.1 ROTI®Dex 50 Medium 100-300 µm 0.5 ml 2-100 µl (50 µl optimal) Proteins >25 kDa,
Oligonucleotides >20 bp,
Nanoparticles >4 nm
25 Pcs
Grav 21AX.1 ROTI®Dex 25 Medium 85-260 µm 1.78 ml 0.15-0.3 ml Proteins >5 kDa,
Oligonucleotides >10 bp,
Nanoparticles >2 nm
50 Pcs
21AY.1 2.75 ml 0.5 ml 50 Pcs
21C0.1 4.31 ml 1 ml 50 Pcs
21C1.1 10.37 ml 2.5 ml 25 Pcs
21C2.1 17.2 ml 5 ml 10 Pcs
21C3.1 34.21 ml 10 ml 10 Pcs
FPLC 21C6.1 ROTI®Dex 25 Superfine 40-110 μm 1 ml 0.05-0.3 ml Proteins >5 kDa,
Oligonucleotides >10 bp,
Nanoparticles >2 nm
5 Pcs
21C6.2 1 ml 0.05-0.3 ml 100 Pcs
21C7.1 5 ml 0.1-1.5 ml 5 Pcs
21C7.2 5 ml 0.1-1.5 ml 25 Pcs
21C7.3 5 ml 0.1-1.5 ml 100 Pcs (4x25)

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Type analysis

Appearancewhite solid
Water absorption2.15-2.25 ml/g
Particle size distribution
50-150 µm (dry)≥80 %
Swelling4-6 ml/g