product.details.print
Proteinase K - Solution, 1 ml, glass
The products marked accordingly are shipped refrigerated in special, sustainable cooling boxes with cooling packs or dry ice.
Sterile products
Ready-to-use products and reagents
Boiling point (bp) ~100 °C
Melting point (mp) ~0 °C
Storage temp. -20 °C
Transport temp. cooled
CAS No. 39450-01-6
EG-Nr. 254-457-8
Non-specific protease for degrading proteins in biological samples.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
29,90 €/op. Campaign price!
plus VAT | 1 ml za op.
Nr kat. 3719.1
Szczegóły dotyczące produktu
Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Foreign activity: RNAse and DNAse not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.
Proteinase K - Solution 20 mg/ml, sterile, ready-to-use, for biochemistry and molecular biology
Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family. Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
- Sterile ready-to-use solution
- Activity ≥ 800 mAnsonU/ ml
- Stable over a wide pH range: 4.0–12.5
- Active at high temperatures and denaturing conditions
- Suma pośrednia: 0.00
| Nr kat. | Op. | Opak. | Cena | Ilość | |
|---|---|---|---|---|---|
| 3719.1 | 1 ml | glass |
39,90 €
29,90 € |
|
|
| 3719.2 | 5 ml | plastic |
159,00 €
119,25 € |
|
|
|
Dostępny z magazynu w Karlsruhe
Dostępne (uwaga na czas dostawy)
Dostępny w późniejszym terminie
Niedostępny
Data dostawy obecnie nieznana
|
|||||
- Suma pośrednia: 0.00
Pobieranie plików / SDS
Informacje ogólne
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
Świadectwa jakości
Guarantee analysis
| Appearance | clear, colorless solution |
| Concentration | 20 mg/ml |
| Specific activity | ≥800 U/ml |
| Assay DNA | ≤200 pg/ml |
| Exonuclease activity | not detectable |
| Endonucleases | not detectable |