Technical Data Sheet
X-ClickSeq Library Prep Kit
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CHF 630.00/Pack Qty.
excl. VAT. | 1 kit per Pack Qty.
Art. No. 3L87.1
Product details
X-ClickSeq Library Prep Kit
ClickSeq is a method for creating NGS libraries that uses click-chemistry instead of enzymes to attach sequencing adapters to cDNA. During reverse transcription, 3'-azido-nucleotides are randomly incorporated, terminating the cDNA strand. These azido-blocked cDNAs are then click-ligated to alkyne-modified adapters and PCR-amplified to create sequencing-ready libraries.X-ClickSeq is the same as ClickSeq, but without provided RT primers, allowing users to apply their own primers based on specific assay needs (e.g., SARS-CoV-2 tiling primers).
- x-Primer Mix
- Click Mix
- Click Accelerant
- Click Catalyst
- i7 Index Primers (Dxxx-Dxxx)
- Elution Buffer 1
- Elution Buffer 2
- No enzymatic ligation required
- Fewer steps, faster workflow
- Extremely low chimera formation (only 3 per million reads)
- Works with both RNA and DNA inputs
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| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
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| 3L87.1 | 1 kit | cardboard |
CHF 630.00 |
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Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
The copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.
References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.
In recent years, click chemistry has become increasingly important and steadily more popular in research and development. The reasons for this are not only the easy handling and the high yields but also the countless application possibilities. Carl ROTH offers you the possibility to purchase the broad portfolio of click reagents, various kits for EdU-based cell proliferation assays and numerous other applications from the well-known brand baseclick. This enables us to offer you a necessary and comprehensive assortment for click chemistry.