Technical Data Sheet
3'-Azido-2'3'-ddGTP, 50 µl
All products identified as being particularly temperaturesensitive are shipped in special ice boxes with freezer packs or in dry ice.
Molar mass (M) 532,20 g/mol
Density (D) ~1 g/cm³
Boiling point (bp) 100 °C
Melting point (mp) 0 °C
Storage temp. -20 °C
Transport temp. cooled
WGK 1
CAS No. 94059-38-8
Azide modified nucleotide closely resemble natural nucleotides and are the basis of chemoenzymatic mRNA labeling. Due to the small size of the azide group, 3'-Azido-2',3'-ddGTP is well accepted by T7 RNA polymerase and poly(A) polymerase and terminale deoxynucleotidyl transferase (Tdt) for enzymatic incorporation. This is highly beneficial, as labeling of mRNA with our approach is modular and not limited to enzymatic compatibility of bulky pre-labeled nucleotides. Moreover, only a very limited number of such pre-modified nucleotides, mainly dye-labeled ones, are commercially available. Internal and external studies successfully showed e.g. that the poly(A) polymerase adds specifically and sequence independently single azido terminator to the 3'-end of RNA.
The main advantage: Any mRNA can be site-specifically labeled without special requirements or altered production protocols at the 3'-end.
€1,064.25/Pack Qty.
excl. VAT. | 50 µl per Pack Qty.
Art. No. 1Y77.2
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
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| 1Y77.1 | 10 µl | plastic |
€311.75 |
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| 1Y77.2 | 50 µl | plastic |
€1,064.25 |
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Downloads / MSDS
General information
The copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.
References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.
In recent years, click chemistry has become increasingly important and steadily more popular in research and development. The reasons for this are not only the easy handling and the high yields but also the countless application possibilities. Carl ROTH offers you the possibility to purchase the broad portfolio of click reagents, various kits for EdU-based cell proliferation assays and numerous other applications from the well-known brand baseclick. This enables us to offer you a necessary and comprehensive assortment for click chemistry.
Nucleotides
Our modified nucleotides for click chemistry complement our range of unmodified standard nucleotides. You can use our alkyne-triphosphates in your nucleotide mixture to incorporate alkyne groups into your PCR fragment. These alkyne groups can be used to label the PCR fragment in a subsequent click reaction.
Certificates of Analysis
Type analysis
| Purity (HPLC) | ≥95 % |