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ROTI®Garose-Glu/GST Cartridges, 5 ml, 1 x 5 ml

ROTI®Garose for biochemistry
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Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H226-H319
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P305+P351+P338
i keep away from heat. No smoking, wear protective gloves/eye protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Packaging
Glutathione-agarose beads, Affinity chromatography colums, Column matrix for protein isolation
Density (D) 0,95 g/cm³
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For isolation of GST-tagged proteins by high pressure affinity chromatography
Suitable for MPLC, FPLC, ÄKTA™ and peristaltic pumps

Glutathione coupled agarose beads for isolation of glutathione-S-transferase (GST) or GST fusion proteins.

ROTI®Garose-Glu/GST Beads offer the perfect solution for your specific application of low flow column chromatography or protein isolation in batch mode, under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.
Product details

€521.40/Pack Qty. 

excl. VAT. | 5 ml per Pack Qty.

Art. No. 0843.1

In production
from 6 Pack Qty. €495.33/Pack Qty.
from 24 Pack Qty. €469.26/Pack Qty.
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Product details


Glutathione coupled agarose beads for isolation of glutathione-S-transferase (GST) or GST fusion proteins.

ROTI®Garose-Glu/GST Beads offer the perfect solution for your specific application of low flow column chromatography or protein isolation in batch mode, under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.

  • Rapid, mild and highly specific purification
  • Suitable for isolation of GST-peptides and protein complexes of all sizes
  • High binding capacity
  • Easy elution and regeneration

Efficient and highly specific isolation of GST-tagged proteins via affinity chromatography is based on the affinity of the glutathion-S-transferase to it’s substrate glutathione. Merely no unspecific binding of proteins without GST-tag takes place, even during purification from raw extracts, while the biological activity of the purified proteins is preserved by chromatography conditions.


Directions for use

The matrix of ROTI®Garose-Glu/GST Beads consists of beaded 4 % agarose without crosslinking, coupled with glutathione. Elution is carried out via a mild Tris/glutathione buffer, enabling even recovery of native protein complexes. ROTI®Garose-Glu/GST Beads may be regenerated, making them very cost-effective.



ROTI®Garose-Glu/GST Cartridges ROTI®Garose for biochemistry

Prepacked cartridges with glutathione coupled agarose beads for medium pressure affinity chromatography (MPLC).
ROTI®Garose-Glu/GST Cartridges offer the perfect solution for your specific application of chromatography under medium pressure (MPLC, FPLC).
Matrix in 20 % ethanol.

  • Easy isolation of GST-tagged proteins from total lysates
  • Recommended flow rate 0,5-5 ml/min.
  • Standard ports (10-32)
  • For medium pressure flow chromatography
  • Very versatile. Applicable with ÄKTA™FPLC™, pump and syringe

Suitable for

Optimal for isolation of glutathione-S-transferase (GST) or GST fusion proteins via automated liquid chromatography, or if proteins shall be isolated under pressure. Suitable for ÄKTA™FPLC™ and for sample application via peristaltic pump or syringe.


Directions for use

ROTI®Garose-Glu/GST Cartridges are prepacked and may be used directly.

Column material made from polypropylene and polyethylene (frit), Inner diameter 1,6 cm, height 2,5 cm, inlet port 10-32 (1/16″) female, outlet port 10-32 (1/16″) male.



Technical Information
Instructions for use, addition Prepacked columns, binding capacity ≥50 mgl/column 
ROTI®Garose-Glu/GST Cartridges
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Art. No. Pack Qty. Pack. Packaging Price Quantity
0843.1 5 ml plastic 1 x 5 ml

€521.40

0843.2 25 ml plastic 5 x 5 ml

€2,101.65

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In procurement
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Selected quantity:   0
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Downloads / MSDS


General information

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Column volume5 ml
Particle size50-150
Beads (un-cross-linked agarose)4 %
LigandGlutathione
Binding capacity/cartridge (GSTrek)≥50 mg