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ROTI®Garose-His/Ni NTA-HPBeads, 25 ml

ROTI®Garose for biochemistry
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H226-H319-EUH208
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P303+P361+P353 P305+P351+P338
i keep away from heat. No smoking, wear protective gloves/eye protection, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Nickel agarose beads, Agarose beads for affinity chromatography, IMAC matrix/nickel charged, Column matrix for protein isolation, IMAC beads
Density (D) 0,95 g/cm³
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For isolation of His-tagged proteins by fast or large scale affinity chromatography under reducing conditions
Suitable for LPLC, FPLC and MPLC and big matrix volumes

For the isolation of His-tagged proteins under reducing conditions.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified.
Product details

€596.65/Pack Qty. 

excl. VAT. | 25 ml per Pack Qty.

Art. No. 0805.1

Available
Ready for dispatch in 3-4 weeks
from 6 Pack Qty. €566.82/Pack Qty.
from 24 Pack Qty. €536.98/Pack Qty.

Product details


For the isolation of His-tagged proteins under reducing conditions.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified.

  • Superior recovery rate of pure His-tagged proteins
  • Minimized Nickel leaching
  • Compatible with denaturing and reducing reagents

The matrix of ROTI®Garose-His/Ni NTA products consists of crosslinked and beaded 6 % agarose, NTA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two histidines that are in close proximity and accessible with good specificity and very high affinity, making the Ni2+ charged matrix the first choice for all standard applications. The tetradentate NTA crosslinker enables highly efficient binding of His-tagged proteins and leads to high recovery rates with minimized nickel bleeding into the eluate.


Directions for use

ROTI®Garose-His/Ni NTA-Beads may be regenerated, making them very cost-effective.
The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride) and (dependent on the respective buffer) reducing substances (for instance ≤30 mM glutathion, ≤10  mM DTT, ≤10 mM DTE, ≤20 mM β-mercaptoethanol und ≤0,3 % SDS).



ROTI®Garose-His/Ni NTA-HPBeads ROTI®Garose for biochemistry

Nickel charged NTA-agarose beads for affinity chromatography under reducing conditions. The matrix of choice for applications under medium pressure, with high flow rate or with big sample-/matrix volume.
In ROTI®Garose-His/Ni NTA-HPBeads, the advantages of the highly efficient Nickel-His binding have been combined with a bead technology allowing a very rapid flow rate. ROTI®Garose-His/Ni HPBeads are ideal for purifying large sample volumes.
50 % bead suspension in 20 % ethanol.

  • Rapid one-step purification of very pure His-tagged proteins from total lysates
  • High binding capacity for 6xHis-tagged protein (≥60 mg/ml purified His-tagged protein)
  • Reliably elution and regeneration
  • For batch mode, gravity flow, MPLC und FPLC
  • Recommended for big matrix volumes

Suitable for

MPLC and FPLC, or if His-tag proteins shall be isolated either in particularly short time or in big amounts from reducing solutions.


Directions for use

May be autoclaved at 121 °C for 30 mins.



Technical Information
Instructions for use, addition Bead suspension, binding capacity ≥60 mg/ml packed matrix (His)6 
ROTI®Garose-His/Ni NTA-HPBeads
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Art. No. Pack Qty. Pack. Price Quantity
0805.1 25 ml plastic

€596.65

0805.2 100 ml plastic

€1,929.65

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Downloads / MSDS


General information

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Particle size50-150 µm
Beads (cross-linked agarose)6 %
LigandNTA
Metal ionnickel
Binding capacity (gel) (GFPuv-6xHis)≥60 mg/ml
Ethanol20 %