ROTI®Garose-Streptavidin Beads, 10 ml
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170
For batch mode and gravity flow
€880.45/Pack Qty.
excl. VAT. | 10 ml per Pack Qty.
Art. No. 0846.2
Now recurring orders conveniently delivered as a subscription!
With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!
How it works:Put all products for your subscription in the desired quantity in the basket.
In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.
Set Starty point and interval for your subscription and submit order!
By the way: Through your account you can customize or delete your subscriptions anytime.
Fits to / Accessories
Product details
ROTI®Garose-Biotin and Streptavidin beads offer the perfect solution for the isolation of biotinylated and streptavidinated molecules in benchtop column chromatography or in batch procedures.
- Rapid, mild and highly specific purification
- Suitable for isolation of DNA, proteins and other molecules of all sizes
- High binding capacity
- High recovery rates
With a value of Ka~10-14/M, the affinity of avidin and streptavidin for biotin is the strongest non-covalent bond between molecules known in biochemistry. Efficient and highly specific isolation of biotinylatd or avidin/streptavidin-tagged molecules via affinity chromatography is based on this particular affinity.
The matrix is easy to pack and results in very evenly packed columns. Elution is carried out via 8 M guanidine-HCl or prior to gel loading during heating in gel loading buffer. Merely no unspecific binding of unlabelled molecules takes place, even during purification from raw extracts.
ROTI®Garose-Streptavidin Beads ROTI®Garose for biochemistry
Biotin coupled agarose beads for affinity chromatography.
50 % bead suspension in 20 % ethanol.
- One-step isolation of biotinylated molecules from total lysates
- For batch mode and gravity flow
Recommended for isolation of biotinylated molecules in batch mode or via columns via low pressure affinity chromatography.
The matrix of ROTI®Garose-Streptavidin Beads consists of beaded 6 % cross-linked agarose. Elution of imminobiotinylated molecules is performed by 50 mM NH4-acetate buffer. Isolation of an antigen by biotinylated antibodies can be performed by 0.1 M glycine buffer.
Streptavidin is immobilised to the beads through a spacer arm and covalent carboxy/amide linkage, not only minimizing streptavidin leakage during elution, but also enhancing the binding capacity by reduction of steric effects.
| Instructions for use, addition | Bead suspension, binding capacity ≥120 nmol/ml packed matrix |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 0846.1 | 5 ml | plastic |
€510.65 |
|
|
| 0846.2 | 10 ml | plastic |
€880.45 |
|
|
|
In stock
In procurement
Not available
Delivery date currently unknown
|
|||||
- Subtotal: 0.00
Downloads / MSDS
General information
Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!
Certificates of Analysis
Type analysis
| Particle size | 50-150 µm |
| Beads (cross-linked agarose) | 6 % |
| Ligand | Streptavidin |
| Binding capacity (gel) (free biotin) | ≥120 nmol/ml |
| Ethanol | 20 % |