ROTI®Garose-Streptavidin Beads, 10 ml
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
For batch mode and gravity flow
excl. VAT. | 10 ml per Pack Qty.
Art. No. 0846.2
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Fits to / Accessories
ROTI®Garose-Biotin and Streptavidin beads offer the perfect solution for the isolation of biotinylated and streptavidinated molecules in benchtop column chromatography or in batch procedures.
- Rapid, mild and highly specific purification
- Suitable for isolation of DNA, proteins and other molecules of all sizes
- High binding capacity
- High recovery rates
With a value of Ka~10-14/M, the affinity of avidin and streptavidin for biotin is the strongest non-covalent bond between molecules known in biochemistry. Efficient and highly specific isolation of biotinylatd or avidin/streptavidin-tagged molecules via affinity chromatography is based on this particular affinity.
The matrix is easy to pack and results in very evenly packed columns. Elution is carried out via 8 M guanidine-HCl or prior to gel loading during heating in gel loading buffer. Merely no unspecific binding of unlabelled molecules takes place, even during purification from raw extracts.
ROTI®Garose-Streptavidin Beads ROTI®Garose for biochemistry
Biotin coupled agarose beads for affinity chromatography.
50 % bead suspension in 20 % ethanol.
- One-step isolation of biotinylated molecules from total lysates
- For batch mode and gravity flow
Recommended for isolation of biotinylated molecules in batch mode or via columns via low pressure affinity chromatography.
The matrix of ROTI®Garose-Streptavidin Beads consists of beaded 6 % cross-linked agarose. Elution of imminobiotinylated molecules is performed by 50 mM NH4-acetate buffer. Isolation of an antigen by biotinylated antibodies can be performed by 0.1 M glycine buffer.
Streptavidin is immobilised to the beads through a spacer arm and covalent carboxy/amide linkage, not only minimizing streptavidin leakage during elution, but also enhancing the binding capacity by reduction of steric effects.
|Instructions for use, addition||Bead suspension, binding capacity ≥120 nmol/ml packed matrix|
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Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!
|Particle size||50-150 µm|
|Beads (cross-linked agarose)||6 %|
|Binding capacity (gel) (free biotin)||≥120 nmol/ml|