ROTI®Garose-Streptavidin Beads, 10 ml
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170
For batch mode and gravity flow
ROTI®Garose-Streptavidin Beads offer the perfect solution for your specific application of low flow column chromatography or isolation in batch mode.
€762.20/Pack Qty.
excl. VAT. | 10 ml per Pack Qty.
Art. No. 0846.2
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Product details
ROTI®Garose-Streptavidin Beads ROTI®Garose for biochemistry
Biotin coupled agarose beads for affinity chromatography. 50 % bead suspension in 20 % ethanol.
ROTI®Garose-Streptavidin Beads offer the perfect solution for your specific application of low flow column chromatography or isolation in batch mode.
- One-step isolation of biotinylated molecules from total lysates
- Rapid, mild and highly specific purification
- Suitable for isolation of DNA, proteins and other molecules of all sizes
- High binding capacity
- High recovery rates
- For batch mode and gravity flow
Recommended for isolation of biotinylated molecules in batch mode or via columns via low pressure affinity chromatography.
The matrix of ROTI®Garose-Streptavidin Beads consists of beaded 6 % cross-linked agarose. The matrix is easy to pack and results in very evenly packed columns. Elution is carried out via 8 M guanidine-HCl or prior to gel loading during heating in gel loading buffer. Elution of imminobiotinylated molecules is performed by 50 mM NH4-acetate buffer. Isolation of an antigen by biotinylated antibodies can be performed by 0.1 M glycine buffer.
With a value of Ka~10-14/M, the affinity of avidin and streptavidin for biotin is the strongest non-covalent bond between molecules known in biochemistry. Efficient and highly specific isolation of biotinylated molecules via affinity chromatography is based on this particular affinity.
Streptavidin is immobilised to the beads through a spacer arm and covalent carboxy/amide linkage, not only minimizing streptavidin leakage during elution, but also enhancing the binding capacity by reduction of steric effects. Merely no unspecific binding of unlabelled molecules takes place, even during purification from raw extracts.
| Instructions for use, addition | Bead suspension, binding capacity ≥120 nmol/ml packed matrix |
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| Order No. | Pack Qty. | Pack. | Price | Quantity | ||
|---|---|---|---|---|---|---|
| 0846.1 | 5 ml | plastic | €446.15 |
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| 0846.2 | 10 ml | plastic | €762.20 |
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- Subtotal: 0.00
Downloads / MSDS
General information
Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!
Certificates of Analysis
Type analysis
| Particle size | 50-150 µm |
| Beads (cross-linked agarose) | 6 % |
| Ligand | Streptavidin |
| Binding capacity (gel) (free biotin) | ≥120 nmol/ml |
| Ethanol | 20 % |