Technical Data Sheet
Methylene blue (C. I. 52015), 100 g, glass
Molar mass (M) 319,9 g/mol
Melting point (mp) 190 °C
WGK 1
CAS No. 61-73-4
EG-Nr. 200-515-2
For microscopy and staining of nucleic acids in polyacrylamide gels.
Application note:
Staining of nucleic acids in PAA
Nucleic acids are visible as blue bands.
Detection limit: 20 ng/band.
€88.15/Pack Qty. Campaign price!
excl. VAT. | 100 g per Pack Qty.
Art. No. A514.3
Product details
Methylene blue (C. I. 52015) pure
Watersoluble nuclear dye, does not intercalate between base pair stacks of DNA. Especially used for staining of blood cells.
Application note:
Staining of nucleic acids in PAA
Nucleic acids are visible as blue bands.
Detection limit: 20 ng/band.
- Use a 0.2 % solution of methylene blue for staining
- Stain for 20 min.
- Wash for 15 min. in distilled water
Dyeing | dead cells blue |
Ox. colour | blue |
Red. colour | colourless |
Redox potential E0[V] at pH=0 and 20 °C | +0.53 (30 °C) |
Use | vitality test |
- Subtotal: 0.00
Art. No. | Pack Qty. | Pack. | Price | Quantity | |
---|---|---|---|---|---|
A514.1 | 10 g | glass |
€24.20
€19.35 |
|
|
A514.2 | 50 g | glass |
€64.40
€51.45 |
|
|
A514.3 | 100 g | glass |
€110.20
€88.15 |
|
|
In stock
Available
In procurement
No longer available
Delivery date currently unknown
|
- Subtotal: 0.00
Downloads / MSDS
General information
Determination of Surfactants
Benzethonium chloride solution is used to measure anionic detergents. In this method, the anionic detergent and the cationic detergent form a 1:1 salt in dichloromethane. The end point of the titration is indicated by methylene blue.
Indicators and Dyes
Carl ROTH offers many indicators and dyes of high purity, which can be used in special applications.
Certificates of Analysis
Type analysis
Appearance | Dark green crystalline powder |
Assay (dry substance) | ≥95 % |
Maximum absorption | 655-670 nm |
Loss on drying (1 h, 100 °C) | ≤10 % |