Rhodamine-12-dUTP, 50 µl, 50 nmol
Molar mass (M) 990,7 g/mol
Boiling point (bp) 100 °C
Melting point (mp) 0 °C
Storage temp. -20 °C
Transport temp. cooled
WGK 1
€348.20/Pack Qty.
excl. VAT. | 50 µl per Pack Qty.
Art. No. 1049.2
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Product details
- DNAse-, RNAse-, Protease- and Phosphatase-free
- Free of PCR inhibitors like modified bases and tetra-pyrophosphate
- Adjusted to pH 7,5 for optimzed enzyme compatibility
- Highly efficient enzymatic fabrication
- Can be optimally combined with Carl ROTH ROTI®Pol DNA polymerases
PCR, cDNA synthesis, labelling and primer-extension, mutagenesis-assays, sequencing reactions and in vitro transcription.
All Carl ROTH nucleotides are manufactured from highest-quality reagents and are most thoroughly tested for quality. This testing procedure not only includes standard-PCR but also “long range PCR”, repeated quantitative light-cycling reactions, and tests for physical stability.
Rhodamine-12-dUTP ≥95 %, 1 mM solution
Rhodamine-12-dUTP is able to replace dTTP in growing DNA-strands and is used for efficient non-radioactive DNA-labelling. Detection of labelled nucleic acids can easily be done by direct analysis of fluorescent signals (e.g. by fluorescence microscopy).
Rhodamine-12-dUTP can also be used for double staining techniques in combination with fluorescein-12-dUTP or biotin-11-dUTP and corresponding antibodies or streptavidin-complexes, respectively.
Excitation: 505 nm
Emission: 530 nm (red)
Non-radioactive labelling of DNA by enzymatic reactions, e.g. PCR, reverse transcription, nick-end-translation, end-labelling or random-primed DNA-labelling. Incorporation can be done with all established DNA-polymerases (e.g. Taq-polymerase, T4 DNA-polymerase, Klenow fragment).
Tested for the lack of endo-, exodeoxyribonuclease, ribonuclease and phosphatase.
Rhodamine Green, mixture of 5/6 isomeres
ε505 (pH 7) = 8,5 E x mmol-1 x cm-1; pH: 7,5 ±0,2
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| Order No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 1049.1 | 25 µl | plastic | 25 nmol | €198.35 |
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| 1049.2 | 50 µl | plastic | 50 nmol | €348.20 |
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