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HPLC column NUCLEODUR® C4 ec, 150x4 mm

From  Macherey-Nagel
Material: stainless steel.
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Wide pore RP phase for daily routine analysis. The medium density butyl modification is based on high purity NUCLEODUR® silica gel and is often used for the separation of biomolecules. Butyl (C4) modifications have a lower retention for hydrophobic compounds compared to C18 (octadecyl silane, ODS) or C8 (octyl, MOS) modifications.
Product details

€379.50/Pack Qty. 

excl. VAT. | 1 unit(s) per Pack Qty.

Art. No. 1HKE.1

Available
Ready for dispatch in 3-4 weeks
from 6 Pack Qty. €360.52/Pack Qty.
from 24 Pack Qty. €341.55/Pack Qty.

Product details

Material: stainless steel.

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!



HPLC column NUCLEODUR® C4 ec 

Wide pore RP phase for daily routine analysis. The medium density butyl modification is based on high purity NUCLEODUR® silica gel and is often used for the separation of biomolecules. Butyl (C4) modifications have a lower retention for hydrophobic compounds compared to C18 (octadecyl silane, ODS) or C8 (octyl, MOS) modifications.



Delivery incl. 1x analytical HPLC column, instruction leaflet, certificate of analysis.
Technical Information
Particle size 5 µm
Column length 150 mm
Column inner diameter 4 mm
Column volume 1.9 ml
Endcapped yes 
Eluent in column Acetonitrile - Water 
Phase C4 ec 
Surface chemistry Medium density monomeric butyl (C4) wide-pore modification 
USP listing L26 
Separation principle Reversed phase (RP) 
Column shape Analytical separation column 
Column type HPLC column 
Particle type Fully porous particles (FPP) 
Specific surface according to BET 100 m²/g
Pore volume 0.9 ml/g
Pore size 300 Å
Temperature stability 60 ° C 
Interactions / retention mechanism hydrophobic (van der Waals interactions), some residual silanol interactions 
Pressure stability 450 bar 
pH stability 1,0-9,0 
Recommended application(s) Biomolecular macromolecules, like proteins and peptides 
Similar phases Jupiter® C4 
ACE® C4 
HPLC column NUCLEODUR® C4 ec
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Art. No. Particle size Pore size Dimensions Pack Qty. Price Quantity
1HKE.1 5 µm 300 Å 150x4 mm 1 unit(s)

€379.50

1HKH.1 5 µm 300 Å 150x4.6 mm 1 unit(s)

€401.00

1HKK.1 5 µm 300 Å 250x4 mm 1 unit(s)

€462.25

1HKL.1 5 µm 300 Å 250x4.6 mm 1 unit(s)

€488.05

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General information

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


NUCLEODUR® Columns for HPLC

NUCLEODUR® as a state-of-the-art silica is the ideal base material for modern HPLC phases. We offer two phases NUCLEODUR® Gravity which is ideal for sophisticated analytical separations and NUCLEODUR® ec suitable for routine HPLC analysis.