Technical Data Sheet
ClickTech EdU Cell Proliferation Kit 555, For 2 x 96 well plate assays.
All products identified as being particularly temperaturesensitive are shipped in special ice boxes with freezer packs or in dry ice.
Ready-to-use products and reagents
Storage temp. +4 °C
Transport temp. cooled
ADR 9 III
WGK 2
UN-Nr. 3082
€344.00/Pack Qty.
excl. VAT. | 1 kit per Pack Qty.
Art. No. 1Y62.1
Product details
With the help of the ClickTech EdU Cell Proliferation Kits, the DNA synthesis of adherent cells can be investigated directly in 96-well plates by means of high throughput screening. Theoretically, this approach can also be carried out with supsension cells, but in this case centrifugation would have to be performed after each incubation or washing step, which would significantly reduce the practicability of this assay.
ClickTech EdU Cell Proliferation Kit 555 for High Throughput Screening
- 5-Ethynyl-deoxyuridine (5-EdU)
- 5-TAMRA-PEG3-Azide
- Reaction buffer
- Reactor System
- Buffer additive
- Rinse buffer (10X)
| λ | 555 nm |
| Markers | 5-TAMRA-PEG3-Azide |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 1Y62.1 | 1 kit | cardboard | For 2 x 96 well plate assays. |
€344.00 |
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| 1Y62.2 | 1 kit | cardboard | For 4 x 96 well plate assays. |
€537.50 |
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In stock
Available
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
The copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.
References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.
In recent years, click chemistry has become increasingly important and steadily more popular in research and development. The reasons for this are not only the easy handling and the high yields but also the countless application possibilities. Carl ROTH offers you the possibility to purchase the broad portfolio of click reagents, various kits for EdU-based cell proliferation assays and numerous other applications from the well-known brand baseclick. This enables us to offer you a necessary and comprehensive assortment for click chemistry.
The number at the end of the product name specifies the excitation wavelength of the included fluorescent marker. Suitable excitation source are:
The ClickTech EdU Cell Proliferation Kits for EdU mediated detection of cell proliferation provide a superior alternative to time-consuming and less sensitive bromodeoxyuridine (BrdU) assays. Similar to BrdU assays, these assays utilise a uracil derivative (in this case ethynyldeoxyuridine, EdU) which is incorporated into the replicating DNA. EdU is not detected via antibodies; instead fluorescent dyes are coupled directly via click reaction. This fast and elegant approach allows for direct labelling of replicating DNA with the desired fluorescent dye. Thus, the assay is perfectly adaptable to the present facilities and to the assay situation (e.g. for double or triple detection). Furthermore, the click reaction is highly selective and therefore prevents any unspecific labelling. The protocol includes only few steps and decreases the total amount of time.
Kits are available for the following applications:
▶ Kits for cell proliferation: • T cell Proliferation (ClickTech EdU T Cell Proliferation Kit - Flow Cytometry) • Fluorescence microscopy (ClickTech EdU Cell Proliferation Kit - Imaging) • High Throughput Screening (ClickTech EdU Cell Proliferation Kit - HTS) • Flow cytometry (ClickTech EdU Cell Proliferation Kit - Flow Cytometry)▶ Kits for nucleic acid modification: • PCR Modification • DNA FISH Kit • RNA Labeling Kit • ClickTech Oligo Link Kit • ClickTech Library Kit - full-length mRNA Seq
Advantages of the click chemistry based approach:
Advantages of the click chemistry based method:
- Highly reliable
- Easy handling
- Fast detection procedure (only 30 min.)
- Mild conditions, no DNA denaturation required
- Modular system in click chemistry
- Compatible with various dyes
- Compatible with multiplexing
| Code in product name | Markers | Absorption (nm) | Emission (nm) | Filter configuration |
| -488 | 6-FAM azide | 496 | 516 | FITC |
| -555 | 5-TAMRA azide | 546 | 579 | Cy3™ |
| -594 | 5/6 Sulforhodamine | 584 | 603 | Texas Red® |
| -647 | Eterneon red (analogous to cyanine 5 azide) | 643 | 662 | Cy5T™ |
The ClickTech EdU Cell Proliferation Kits for High Throughput Screening allow the measurement of DNA synthesis adherent cells in 96-well plate format. Similar to BrdU assays, these assays utilise an uracil derivative (in this case ethynyldeoxyuridine, EdU) which is incorporated into the replicating DNA. EdU is not detected via antibodies; instead fluorescent dyes are coupled directly via click reaction. This fast and elegant approach allows for direct labelling of replicating DNA with the desired fluorescent dye. Thus, the assay is perfectly adaptable to the present facilities. Furthermore, the click reaction is highly selective and therefore prevents any unspecific labelling. The protocol includes only few steps and decreases the total amount of time.
The ClickTech EdU Cell Proliferation Kits for High Throughput Screening allow the measurement of DNA synthesis adherent cells in 96-well plate format. Similar to BrdU assays, these assays utilise an uracil derivative (in this case ethynyldeoxyuridine, EdU) which is incorporated into the replicating DNA. EdU is not detected via antibodies; instead fluorescent dyes are coupled directly via click reaction. This fast and elegant approach allows for direct labelling of replicating DNA with the desired fluorescent dye. Thus, the assay is perfectly adaptable to the present facilities. Furthermore, the click reaction is highly selective and therefore prevents any unspecific labelling. The protocol includes only few steps and decreases the total amount of time.