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Transfer membrane ROTI®PVDF 0.2
From  Carl ROTH
Material: polyvinylidene fluoride (PVDF).
Related products
Dimensions


PVDF membrane for protein analyses.

ROTI®PVDF is a hydrophobic, microporous polyvinylidene membrane, free from adjuvants and detergents. With this membrane, efficient transfers of proteins can be carried out over a wide molecular weight range.
Product details

€451.50/Pack Qty. 

excl. VAT. | 1 roll(s) per Pack Qty.

Art. No. 1YYC.1

In stock
Delivery fast, simple and reliable!
from 6 Pack Qty. €428.92/Pack Qty.
from 24 Pack Qty. €406.35/Pack Qty.

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Product details

Material: polyvinylidene fluoride (PVDF).

PVDF membrane for protein analyses.

ROTI®PVDF is a hydrophobic, microporous polyvinylidene membrane, free from adjuvants and detergents. With this membrane, efficient transfers of proteins can be carried out over a wide molecular weight range.


Directions for use

The membrane shows superior protein binding parameters and stands out for its particularly low background. It may also be used very well after repeated stripping and harsh washing processes.
This mechanically rigid transfer membrane can be easily cut to the desired gel size and it remains flexible and tear-proof even when dry.
Briefly immerse the membrane membrane in 100 % methanol prior to transfer until no more white areas are visible. This transfer membrane is then compatible with every blotting system.
Due to production processes, the membrane shows two different sides - one smooth and one raw. For optimally distinct bands, please use the smooth side for protein binding.



Transfer membrane ROTI®PVDF 0.2 
Suitable for

Optimal for proteins≤70 kDa.
Detection by Chemoluminescence, radioactivity, colour.

Applications:
  • Western blotting
  • Immuno-blotting
  • Solid phase assays
  • Amino acid or protein analysis


Not a medical device / Not an IVD product
Technical Information
Dimensions 300 × 30 cm 
Version Roll 
Pore size 0,2 µm
Bond hydrophobic 
Binding capacity 125 µg/cm²
Thickn. 190±50 µm
Immobilisation not necessary 
Transfer membrane ROTI®PVDF 0.2
Selected quantity:   0
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Art. No. Dimensions Pack Qty. Price Quantity
1YYC.1 300 × 30 cm 1 roll(s)

€451.50

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Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS

No documents available

General information

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Roti® PVDF 0.2 Polyvinyliden fluoride hydrophobic 140 ±20 ≥125 0,2 not necessary
Roti® PVDF Polyvinyliden fluoride hydrophobic 125 ±25 ca. 200 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Roti® NC Nitrocellulose electrostatic, hydrophobic 150 ±50 125-200 0,2 UV, baking (max. 80 °C)
Roti® NC 0.45 Nitrocellulose electrostatic, hydrophobic 150 ±10 ≥125 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not