Transfer membrane ROTI®PVDF 0.2
ROTI®PVDF is a hydrophobic, microporous polyvinylidene membrane, free from adjuvants and detergents. With this membrane, efficient transfers of proteins can be carried out over a wide molecular weight range.
€451.50/Pack Qty.
excl. VAT. | 1 roll(s) per Pack Qty.
Art. No. 1YYC.1
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Product details
PVDF membrane for protein analyses.
ROTI®PVDF is a hydrophobic, microporous polyvinylidene membrane, free from adjuvants and detergents. With this membrane, efficient transfers of proteins can be carried out over a wide molecular weight range.
The membrane shows superior protein binding parameters and stands out for its particularly low background. It may also be used very well after repeated stripping and harsh washing processes.
This mechanically rigid transfer membrane can be easily cut to the desired gel size and it remains flexible and tear-proof even when dry.
Briefly immerse the membrane membrane in 100 % methanol prior to transfer until no more white areas are visible. This transfer membrane is then compatible with every blotting system.
Due to production processes, the membrane shows two different sides - one smooth and one raw. For optimally distinct bands, please use the smooth side for protein binding.
Transfer membrane ROTI®PVDF 0.2
Optimal for proteins≤70 kDa.
Detection by Chemoluminescence, radioactivity, colour.
- Western blotting
- Immuno-blotting
- Solid phase assays
- Amino acid or protein analysis
Dimensions | 300 × 30 cm |
Version | Roll |
Pore size | 0,2 µm |
Bond | hydrophobic |
Binding capacity | 125 µg/cm² |
Thickn. | 190±50 µm |
Immobilisation | not necessary |
- Subtotal: 0.00
Art. No. | Dimensions | Pack Qty. | Price | Quantity | |
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1YYC.1 | 300 × 30 cm | 1 roll(s) |
€451.50 |
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In stock
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
Technical Parameters of ROTH-Transfer Membranes
Membrane | Material | Characteristics | Thickness (μm) | Binding capacity (μg/cm2) | Pore size (μm) | Immobilisation |
Roti® Fluoro PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | 300 | 0,2 | not necessary |
Roti® PVDF 0.2 | Polyvinyliden fluoride | hydrophobic | 140 ±20 | ≥125 | 0,2 | not necessary |
Roti® PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | ca. 200 | 0,45 | not necessary |
Immobilon P | Polyvinyliden fluoride | hydrophobic | 120 ±25 | 150-300 | 0,45 | not necessary |
Roti® Nylon 0.2 | Nylon 6,6 | neutral/amphoteric | 150 ±20 | 500 | 0,2/0,45 | UV, baking |
Roti® Nylon plus | Nylon 6,6 | hydrophilic, positively charged | 160 ±20 | 500 | 0,45 | not necessary, UV and baking possible |
Roti® NC | Nitrocellulose | electrostatic, hydrophobic | 150 ±50 | 125-200 | 0,2 | UV, baking (max. 80 °C) |
Roti® NC 0.45 | Nitrocellulose | electrostatic, hydrophobic | 150 ±10 | ≥125 | 0,45 | UV, baking (max. 80 °C) |
Amersham Protran® | Nitrocellulose | electrostatic, hydrophobic | unknown | 11-72 | 0,2/0,45 | UV, baking (max. 80 °C) |
Amersham Protran® supported | Nitrocellulose | electrostatic, hydrophobic | unknown | 86-110 | 0,2/0,45 | UV, baking (max. 80 °C) |
Biomolecules / Membrane Pores | Size (kD) |
genomic DNA (human) | >11000000 |
E. coli | approx. 1500000 |
0.45 μm | approx. 5000 |
0.2 μm | approx. 2000 |
DNA 3 kb | approx. 1000 |
RNA 1 kb | approx. 300 |
Antibodies (IgG) | approx. 150 |
Proteins in the lab | approx. 10–300 |
BSA (Albumin) | 69 |
Biomolecules | Nylon plus | Nylon 0.2 | PVDF 0.2 | PVDF | Fluoro PVDF | NC | NC 0.45 |
DNA | +++ | + | - | - | - | + | + |
RNA | + | +++ | - | - | - | + | + |
Proteins | - | + | +++ | +++ | +++ | + | + |
Detection System | |||||||
Colour | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
Radioactivity | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
Chemoluminescence | +++ | +++ | +++ | +++ | - | + | + |
Fluorescence | +++ | +++ | - | - | +++ | - | - |
+++: recommended | +: suitable | -: not |