Jump to content Jump to navigation menu

HPLC column NUCLEODUR® NH2-RP 5 µm, 250 mm, 4.6 mm

From  Macherey-Nagel
Material: stainless steel.
Particle size
Column length
Column inner diameter


This HPLC column is packed with polar aminopropyl modification, based on high purity NUCLEODUR® silica gel. This HPLC phase is able to generate a clearly recognizable different retention behavior compared to purely alkyl-functionalized surface modifications. Besides cyano modifications, amino modifications belong to the most frequently used polar silica phases. Amino phases can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. Also anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile) is possible. Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions.
Product details

€488.05/Pack Qty. 

excl. VAT. | 1 unit(s) per Pack Qty.

Art. No. 1NEA.1

Available
Ready for dispatch in 3-4 weeks
from 6 Pack Qty. €463.65/Pack Qty.
from 24 Pack Qty. €439.24/Pack Qty.

Product details

Material: stainless steel.

This HPLC column is packed with polar aminopropyl modification, based on high purity NUCLEODUR® silica gel. This HPLC phase is able to generate a clearly recognizable different retention behavior compared to purely alkyl-functionalized surface modifications. Besides cyano modifications, amino modifications belong to the most frequently used polar silica phases. Amino phases can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. Also anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile) is possible. Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions.


Applications:
Hydrocarbons under NP conditions, Polar compounds under RP conditions (sugars, DNA bases), USP L8, suitable for LC/MS.



Similar phases:
NUCLEOSIL® NH2 / NH2-RP


Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


For protection of your main HPLC column from contamination, it is recommended to use guard columns. These short columns are placed into a guard column holder. The guard column holder in combination with a guard column inside is installed between the injector and the HPLC column. This installation prevents chemical impurities to reach the main column. Because a contamination could form an irreversible bond with the column packing material (stationary phase) and this can result in shorter column life. If you want to prolong the lifetime of your main column, then these short columns can help you.


Further HPLC columns from Macherey-Nagel available on request.


HPLC Columns NUCLEODUR® “Gravity”

The ideal phase for overall sophisticated analytical separations

This high density phase with its endcapping suppresses any unwanted polar interactions between the silica surface and the sample, which makes “Gravity” particularly suitable for the separation of basic and other ionizable analytes.

Properties:

  • Suitable for LC/MS and HPLC at pH extremes (pH 1-11)
  • Ideal for method development
  • Superior base deactivation
  • Multi-Endcapping


Recommended application(s):

  • In general, compounds with ionisable functional groups such as basic pharmaceuticals and pesticides e.g. analgesics, anti-inflammatories, antidepressants, herbicides, phytopharmaceuticals, immunosuppressants.
  • USP L1 (C18) · USP L7 (C8)



HPLC column NUCLEODUR® NH2-RP 5 µm 

Technical Information
Particle size 5 µm
Column length 250 mm
Column inner diameter 4.6 mm
Column volume 4.15 ml
Endcapped no 
Eluent in column Acetonitrile - Water 
Phase NH2-RP 
Surface chemistry Aminopropyl (NH2) modification 
USP listing L8 
Separation principle Multi-mode (RP and NP), Reversed phase (RP) 
Column shape Analytical separation column 
Column type HPLC column 
Particle type Fully porous particles (FPP) 
Specific surface according to BET 340 m²/g
Pore volume 0.9 ml/g
Pore size 110 Å
Temperature stability 60 ° C 
Pressure stability 600 bar 
pH stability 2,0-8,0 
Recommended application(s) Polar compounds (sugars, DNA bases) 
Similar phases NUCLEOSIL® NH2 / NH2-RP 
HPLC column NUCLEODUR® NH2-RP 5 µm
Selected quantity:   0
  1. Subtotal:  0.00
Art. No. Particle size Column length Column inner diameter Pack Qty. Price Quantity
1NE6.1 5 µm 125 mm 4 mm 1 unit(s)

€379.50

1NE7.1 5 µm 125 mm 4,6 mm 1 unit(s)

€401.00

1NE8.1 5 µm 150 mm 4,6 mm 1 unit(s)

€401.00

1NE9.1 5 µm 250 mm 4 mm 1 unit(s)

€462.25

1NEA.1 5 µm 250 mm 4,6 mm 1 unit(s)

€488.05

In stock
Available
In procurement
No longer available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS

No documents available

General information

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


NUCLEODUR® Columns for HPLC

NUCLEODUR® as a state-of-the-art silica is the ideal base material for modern HPLC phases. We offer two phases NUCLEODUR® Gravity which is ideal for sophisticated analytical separations and NUCLEODUR® ec suitable for routine HPLC analysis.