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HPLC column NUCLEODUR® 100-5 C18 ec 5 µm, 250 mm, 4.6 mm

From  Macherey-Nagel
Material: stainless steel.
Particle size
Column length
Column inner diameter

The ideal phase for routine HPLC analysis.
Product details

€488.05/Pack Qty. 

excl. VAT. | 1 unit(s) per Pack Qty.

Art. No. KK85.1

Ready for dispatch in 3-4 weeks
from 6 Pack Qty. €463.65/Pack Qty.
from 24 Pack Qty. €439.24/Pack Qty.

Product details

Material: stainless steel.

The ideal phase for routine HPLC analysis.

  • Octadecyl phase, endcapped
  • Carbon content: 17.5 %
  • Pore size: 110 Å
  • pH stability: 1-9
  • Eluent on column: acetonitrile/water
Eluent: acetonitril/water (75/25, v/v); Flow: 1,3 ml/min; Temperature: 30 °C; Detection: UV, 254 nm

1. Toluene, 2. Ethylbenzene, 3. Propylbenzene, 4. Butylbenzene, 5. Pentylbenzene

Similar phases:
For many applicatons the following phases can be replaced with identical or even better results: NUCLEOSIL® C18, Spherisorb® ODS I und II, Hypersil™ ODS, Waters Symmetry®C18, Inertsil® ODS II, Kromasil® C18, LiChrospher® RP18 und RP C18 ec.

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!

For protection of your main HPLC column from contamination, it is recommended to use guard columns. These short columns are placed into a guard column holder. The guard column holder in combination with a guard column inside is installed between the injector and the HPLC column. This installation prevents chemical impurities to reach the main column. Because a contamination could form an irreversible bond with the column packing material (stationary phase) and this can result in shorter column life. If you want to prolong the lifetime of your main column, then these short columns can help you.

Further HPLC columns from Macherey-Nagel available on request.


Non-polar phases for routine analysis

Standard RP phase (110 Å) for daily routine analysis and up-scaling to preparative HPLC. Also available wide-pore (300 Å) for separation of biomolecules in analytical columns.


  • Cost-effective and reliable
  • Long-life columns
  • For all standard routine applications in modern RP-chromatography

Recommended application(s):

  • Pore size 100 Å:
    Basic, neutral and acidic pharmaceuticals, derivatised amino acids, pesticides, fat-soluble vitamins, aldehydes and ketones, phenolic compounds.
  • Pore size 300 Å:
    Biological macromolecules, such as proteins or peptides.
  • USP L1 (C18) · USP L7 (C8) · USP L26 (C4)

HPLC column NUCLEODUR® 100-5 C18 ec 5 µm 

Technical Information
Particle size 5 µm
Column length 250 mm
Column inner diameter 4.6 mm
Column volume 4.15 ml
Endcapped yes 
Eluent in column Acetonitrile - Water 
Phase 100-5 C18 ec 
Surface chemistry Medium density monomeric octadecyl (C18, ODS) modification 
USP listing L1 
Separation principle Reversed phase (RP) 
Column shape Analytical separation column 
Column type HPLC column 
Particle type Fully porous particles (FPP) 
Specific surface according to BET 340 m²/g
Pore volume 0.9 ml/g
Pore size 110 Å
Temperature stability 60 °C 
Pressure stability 600 bar 
pH stability 1,0-9,0 
Recommended application(s) Aldehydes, Basic, neutral or acidic drugs, Derivatized amino acids, Fat-soluble vitamins, Ketones, Pesticides, Phenolic compounds 
Similar phases NUCLEOSIL® C18 
Spherisorb® ODS I 
Spherisorb® ODS II 
Hypersil™ ODS 
Symmetry® C18 
Inertsil® ODS II 
Kromasil® C18 
LiChrospher® RP18 
LiChrospher® RP C18 ec 
HPLC column NUCLEODUR® 100-5 C18 ec 5 µm
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Art. No. Particle size Column length Column inner diameter Pack Qty. Price Quantity
KK85.1 5 µm 250 mm 4,6 mm 1 unit(s)


KK84.1 5 µm 150 mm 4,6 mm 1 unit(s)


KK83.1 5 µm 250 mm 4 mm 1 unit(s)


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Downloads / MSDS

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General information

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


NUCLEODUR® as a state-of-the-art silica is the ideal base material for modern HPLC phases. We offer two phases NUCLEODUR® Gravity which is ideal for sophisticated analytical separations and NUCLEODUR® ec suitable for routine HPLC analysis.

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