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HPLC column NUCLEOSHELL® Phenyl-Hexyl, 100 mm, 4 mm

From  Macherey-Nagel
Material: stainless steel.
Particle size
Column length
Column inner diameter

Phenylhexyl modification, multi-endcapped
Product details

€817.00/Pack Qty. 

excl. VAT. | 1 unit(s) per Pack Qty.

Art. No. 21Y9.1

Ready for dispatch in 3-4 weeks
from 6 Pack Qty. €776.15/Pack Qty.
from 24 Pack Qty. €735.30/Pack Qty.

Product details

Material: stainless steel.

Phenylhexyl modification, multi-endcapped

  • Core-shell technology for fast and efficient HPLC
  • Hydrophobic phase with an alternative selectivity compared to classical C18 modifications
  • Separation principle based on 2 retention mechanisms: π-π interactions and hydrophobic interactions

General properties:
  • Pore size 90 Å
  • Particle size 2.7 μm
  • Carbon content 4.5
  • pH stability 1-10; suitable for LC/MS

NUCLEOSHELL® Phenyl-Hexyl is a robust phase with alternative RP selectivity for aromatic and unsaturated analytes compared to classical C18 / C8 phases - making it a useful additional tool for all chromatography users.

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!

For protection of your main HPLC column from contamination, it is recommended to use guard columns. These short columns are placed into a guard column holder. The guard column holder in combination with a guard column inside is installed between the injector and the HPLC column. This installation prevents chemical impurities to reach the main column. Because a contamination could form an irreversible bond with the column packing material (stationary phase) and this can result in shorter column life. If you want to prolong the lifetime of your main column, then these short columns can help you.

Further HPLC columns from Macherey-Nagel available on request.


Non-polar phases for routine analysis

Standard RP phase (110 Å) for daily routine analysis and up-scaling to preparative HPLC. Also available wide-pore (300 Å) for separation of biomolecules in analytical columns.


  • Cost-effective and reliable
  • Long-life columns
  • For all standard routine applications in modern RP-chromatography

Recommended application(s):

  • Pore size 100 Å:
    Basic, neutral and acidic pharmaceuticals, derivatised amino acids, pesticides, fat-soluble vitamins, aldehydes and ketones, phenolic compounds.
  • Pore size 300 Å:
    Biological macromolecules, such as proteins or peptides.
  • USP L1 (C18) · USP L7 (C8) · USP L26 (C4)

HPLC column NUCLEOSHELL® Phenyl-Hexyl 

Technical Information
Particle size 2,7 µm
Column length 100 mm
Column inner diameter 4 mm
Column volume 1.25 ml
Endcapped yes 
Eluent in column Acetonitrile - Water 
Phase Phenyl-Hexyl 
Surface chemistry Phenylhexyl modification 
USP listing L11 
Separation principle Reversed phase (RP) 
Column shape Analytical separation column 
Column type HPLC column 
Particle type Core-shell, superficially porous particles (SPP) 
Specific surface according to BET 130 m²/g
Pore size 90 Å
Temperature stability 60 °C 
Pressure stability 600 bar 
pH stability 1,0-10,0 
Recommended application(s) Antibiotics 
Aromatic and unsaturated compounds 
Especially for polar compounds 
HPLC column NUCLEOSHELL® Phenyl-Hexyl
Selected quantity:   0
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Art. No. Particle size Column length Column inner diameter Pack Qty. Price Quantity
21Y3.1 2,7 µm 50 mm 2 mm 1 unit(s)


21Y4.1 2,7 µm 50 mm 3 mm 1 unit(s)


21Y5.1 2,7 µm 50 mm 4 mm 1 unit(s)


21Y6.1 2,7 µm 50 mm 4,6 mm 1 unit(s)


21Y7.1 2,7 µm 100 mm 2 mm 1 unit(s)


21Y8.1 2,7 µm 100 mm 3 mm 1 unit(s)


21Y9.1 2,7 µm 100 mm 4 mm 1 unit(s)


21YA.1 2,7 µm 100 mm 4,6 mm 1 unit(s)


21YC.1 2,7 µm 150 mm 2 mm 1 unit(s)


21YE.1 2,7 µm 150 mm 3 mm 1 unit(s)


21YH.1 2,7 µm 150 mm 4 mm 1 unit(s)


21YK.1 2,7 µm 150 mm 4,6 mm 1 unit(s)


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Downloads / MSDS

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General information

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


NUCLEODUR® as a state-of-the-art silica is the ideal base material for modern HPLC phases. We offer two phases NUCLEODUR® Gravity which is ideal for sophisticated analytical separations and NUCLEODUR® ec suitable for routine HPLC analysis.