Technical Data Sheet
Brilliant blue R 250 (C.I. 42660), 100 g
for electrophoresis
Pack Qty.
Pack.
Coomassie® blue R 250
Empirical formula C45H44N3NaO7S2
Molar mass (M) 825,99 g/mol
Density (D) 1 g/cm³
Melting point (mp) 180 °C
WGK 1
CAS No. 6104-59-2
EG-Nr. 228-060-5
Molar mass (M) 825,99 g/mol
Density (D) 1 g/cm³
Melting point (mp) 180 °C
WGK 1
CAS No. 6104-59-2
EG-Nr. 228-060-5
Conditionally suitable for staining of membranes after protein transfer.
For preparing protein gel staining solutions
For preparing protein gel staining solutions
€131.70/Pack Qty.
excl. VAT. | 100 g per Pack Qty.
Art. No. 3862.2
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Product details
Brilliant blue R 250 (C.I. 42660) for electrophoresis
Technical Information
| Sensitivity | 50 |
| Use | Dye for performing Coomassie® staining. ODmax = 550 |
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| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 3862.1 | 10 g | glass |
€32.80 |
|
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| 3862.2 | 100 g | glass |
€131.70 |
|
|
|
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Downloads / MSDS
General information
Suitability of Protein Stainings
| Product | Sensitivity | Rapid Gel Analysis |
Gel Drying |
Elution/ Transfer |
2D- Gels |
MS- Analysis |
| ROTI®Black P | 1 ng/mm2 | - | +++ | - | +++ | - |
| ROTI®Blue | 10 ng/mm2 | - | +++ | +++ | - | +++ |
| ROTI®Blue quick | 10 ng/mm2 | +++ | +++ | ++ | - | ++ |
| ROTI®Blau R | 50 ng/mm2 | +++ | + | - | - | - |
| +++: recommended +: suitable -: not recommended | ||||||
Summary Staining of Protein Gels
| Product | Sensitivity | Description |
| ROTI®Black P | 1 ng/mm2 | Silver staining kit. Highly sensitive. Simple, fast and reproducible. |
| ROTI®Blue | 10 ng/mm2 | Coomassie®-solution for colloidal staining of proteins. Destaining not required. Non-fixing staining for protein gel elution possible. Proteomics-compatible. |
| ROTI®Blue quick | 10 ng/mm2 | Solution for rapid, highly specific staining of proteins. Destaining not required. Non-fixing staining, compatible with blotting and Proteomics. |
| ROTIPHORESE®Blau R | 50 ng/mm2 | Coomassie® staining solution concentrate for rapid staining. |
| Coomassie® Blue G250 (C.I. 42655) | 60 ng/mm2 | Dye for performing Coomassie® staining. ODmax = 595 |
| Coomassie® Blue R250 (C.I. 42660) | 50 ng/mm2 | Dye for performing Coomassie staining. ODmax = 550 |
Certificates of Analysis
Type analysis
| Appearance | blue to purple powder |
| Absorption max. (in EtOH) | 580-600 nm |
| Loss on drying (110 °C) | ≤10 % |