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ROTI®Garose-His/Ni NTA-Cartridges, 1 x 5 ml
Ready-to-use products and reagents
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170
Matrix volume 5 ml, whole volume 10 ml. For approx. 250 mg His labelled protein.
For isolation of His-tagged proteins by high pressure affinity chromatography under reducing conditions
Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified.
274,15 €/VE
Excl. btw | 5 ml Per VE
Bestelnr. 0804.1
Geschikt voor / Toebehoren
Productdetails
For the isolation of His-tagged proteins under reducing conditions.
Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified.
- Superior recovery rate of pure His-tagged proteins
- Minimized Nickel leaching
- Compatible with denaturing and reducing reagents
The matrix of ROTI®Garose-His/Ni NTA products consists of crosslinked and beaded 6 % agarose, NTA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two histidines that are in close proximity and accessible with good specificity and very high affinity, making the Ni2+ charged matrix the first choice for all standard applications. The tetradentate NTA crosslinker enables highly efficient binding of His-tagged proteins and leads to high recovery rates with minimized nickel bleeding into the eluate.
ROTI®Garose-His/Ni NTA-Beads may be regenerated, making them very cost-effective.
The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride) and (dependent on the respective buffer) reducing substances (for instance ≤30 mM glutathion, ≤10 mM DTT, ≤10 mM DTE, ≤20 mM β-mercaptoethanol und ≤0,3 % SDS).
ROTI®Garose-His/Ni NTA-Cartridges ROTI®Garose for biochemistry
Prepacked cartridges with Nickel charged NTA-agarose bead matrix for medium pressure affinity chromatography (MPLC) under reducing conditions.
Immobilized metal ion affinity chromatography (IMAC) under medium pressure is the prefect method to obtain high yield of very pure proteins in minimum time. ROTI®Garose-His/Ni NTA-Cartridges offer the perfect solution for fast and simple isolation of His-tagged proteins from total lysates in reducing buffer solutions.
Matrix in 15 % ethanol.
- Very fast and simple purification of His-tagged proteins from total lysates
- High binding capacity for 6×His-tagged protein
- Recommended flow rate 5 ml/min
- Standard ports 10-32 (1/16″)
- Very versatile. Applicable with ÄKTA™FPLC™, pump and syringe
- Reliable elution and regeneration
- For medium pressure flow chromatography
ÄKTA™FPLC™ and for sample application via peristaltic pump or syringe.
ROTI®Garose His/Ni NTA-Cartridges are prepacked and may be used directly.
Column material made from polypropylene and polyethylene (frit), Inner diameter 1,6 cm, height 2,5 cm, inlet port 10-32 (1/16″) female, outlet port 10-32 (1/16″) male.
| Gebruikstip | Prepacked columns, binding capacity 50-250 mg/column (His)6 |
- Tussentotaal: 0.00
| Bestelnr. | VE | Verp. | Verpakking | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|---|
| 0804.1 | 5 ml | plastic | 1 x 5 ml |
274,15 € |
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| 0804.2 | 5 ml | plastic | 5 x 5 ml |
994,40 € |
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
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Analysecertificaten
Type analysis
| Column volume | 5 ml |
| Particle size | 50-150 µm |
| Beads (cross-linked agarose) | 6 % |
| Ligand | NTA |
| Metal ion | nickel |
| Binding capacity/cartridge (GFPuv-6xHis) | 250 mg |