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ROTI®Garose-Streptavidin Beads, 5 ml
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170
For isolation of biotin-coupled molecules by affinity chromatography
521,40 €/VE
Excl. btw | 5 ml Per VE
Bestelnr. 0846.1
Geschikt voor / Toebehoren
Productdetails
ROTI®Garose-Biotin and Streptavidin beads offer the perfect solution for the isolation of biotinylated and streptavidinated molecules in benchtop column chromatography or in batch procedures.
- Rapid, mild and highly specific purification
- Suitable for isolation of DNA, proteins and other molecules of all sizes
- High binding capacity
- High recovery rates
With a value of Ka~10-14/M, the affinity of avidin and streptavidin for biotin is the strongest non-covalent bond between molecules known in biochemistry. Efficient and highly specific isolation of biotinylatd or avidin/streptavidin-tagged molecules via affinity chromatography is based on this particular affinity.
The matrix is easy to pack and results in very evenly packed columns. Elution is carried out via 8 M guanidine-HCl or prior to gel loading during heating in gel loading buffer. Merely no unspecific binding of unlabelled molecules takes place, even during purification from raw extracts.
ROTI®Garose-Streptavidin Beads ROTI®Garose for biochemistry
Biotin coupled agarose beads for affinity chromatography. 50 % bead suspension in 20 % ethanol.
ROTI®Garose-Streptavidin Beads offer the perfect solution for your specific application of low flow column chromatography or isolation in batch mode.
- One-step isolation of biotinylated molecules from total lysates
- For batch mode and gravity flow
Recommended for isolation of biotinylated molecules in batch mode or via columns via low pressure affinity chromatography.
The matrix of ROTI®Garose-Streptavidin Beads consists of beaded 6 % cross-linked agarose. Elution of imminobiotinylated molecules is performed by 50 mM NH4-acetate buffer. Isolation of an antigen by biotinylated antibodies can be performed by 0.1 M glycine buffer.
Streptavidin is immobilised to the beads through a spacer arm and covalent carboxy/amide linkage, not only minimizing streptavidin leakage during elution, but also enhancing the binding capacity by reduction of steric effects.
| Gebruikstip | Bead suspension, binding capacity ≥120 nmol/ml packed matrix |
- Tussentotaal: 0.00
| Bestelnr. | VE | Verp. | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|
| 0846.1 | 5 ml | plastic |
521,40 € |
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| 0846.2 | 10 ml | plastic |
901,95 € |
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Op voorraad
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
U vindt nog meer aantrekkelijke aanbiedingen om uw chromatografielaboratorium te vervolledigen op onze Chromatografie pagina!
Analysecertificaten
Type analysis
| Particle size | 50-150 µm |
| Beads (cross-linked agarose) | 6 % |
| Ligand | Streptavidin |
| Binding capacity (gel) (free biotin) | ≥120 nmol/ml |
| Ethanol | 20 % |