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Lysozyme, 25 g

≥45 000 FIP U/mg, lyophilized
Mogelijke gevolgen: veroorzaken allergieën, kankerverwekkend (carcinogeen), verandering erfelijk materiaal (mutageen), brengt de voortplanting in gevaar en vruchtbeschadigend (reprotoxisch) of orgaanbeschadigend Veiligheid: voor het werk met dergelijke stoffen moet men zich goed informeren; veiligheidskleding en handschoenen, oog- en mondbescherming of adembescherming dragen.
Danger
H334
i may cause allergy or asthma symptoms or breathing difficulties if inhaled
P260 P342+P311
i do not breathe dust, if experiencing respiratory symptoms: Call a POISON CENTER/doctor
VE
Verp.
Molar mass (M) ~14000 g/mol
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
CAS No. 12650-88-3
EG-Nr. 235-747-3

From chicken egg white, free from albumin.
Stock solution: 10-50 mg/ml in 10 mM Tris (pH 8,0), prepare directly before use

Salt and albumin free lyophilised powder. Quality product for molecular biology. Ideal for lysis of bacteria for preparing DNA.

Lysozyme preferentially hydrolyses the β-1,4-glycosidic bond between N-acetylmuraminic acid and N-acetylglucosamine. Muramic acid and acetylglucosamine are components of the proteoglycan cell wall of many microorganisms. The enzyme is mostly derived from chicken egg white and may efficiently be used for lysis of Gram+ bacteria. In genetic engineering, it is very often used during isolation of plasmid DNA or cellular RNA for the lysis of E. coli or eukaryotic cells.
Productdetails

392,40 €/VE 

Excl. btw | 25 g Per VE

Bestelnr. 8259.3

Op voorraad
Vrij van verzendingskosten vanaf 250 €
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Vanaf 6 VE 372,78 €/VE
Vanaf 24 VE 353,16 €/VE

Productdetails



Lysozyme ≥45 000 FIP U/mg, lyophilized

Salt and albumin free lyophilised powder. Quality product for molecular biology. Ideal for lysis of bacteria for preparing DNA.

Lysozyme preferentially hydrolyses the β-1,4-glycosidic bond between N-acetylmuraminic acid and N-acetylglucosamine. Muramic acid and acetylglucosamine are components of the proteoglycan cell wall of many microorganisms. The enzyme is mostly derived from chicken egg white and may efficiently be used for lysis of Gram+ bacteria. In genetic engineering, it is very often used during isolation of plasmid DNA or cellular RNA for the lysis of E. coli or eukaryotic cells.


Unit-definitie

1 FIP-Unit equals the amount of enzyme which reduces the absorbance by 0.001 per min. at 450 nm, 25 °C, pH 7.00 using a suspension of FIP Micrococcus luteus as substrate (acc. to Pharmaceutical Enzymes 1997). 1 FIP-Unit resembles approximately 1 Shugar-Unit.


Gebruiksinstructies

Working solution: 5-10 mg/ml (bacteria), 1-2 mg/ml (eukaryotic cells)
Buffer: salt solution without detegents (0.1 M potassium phosphate buffer, 50 mM Tris buffer, 1x PBS, 1x TBS or similar)
pH optimum: 6.0-7.0
Inhibitors: imidazole, indole, N-acetyl glucosamine
Temperature optimum: 37 °C
Stability: The lyophilisate is stable at +4 °C and -20 °C for several years. The stability of the solution at pH 4-5 is several weeks at +4 °C and several days at room temperature.
Isoelectric point: approx. 11
Lysozyme solution should be frozen only once.



Lysozyme
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Bestelnr. VE Verp. Prijs Hoeveelheid
8259.1 1 g glass

38,20 €

8259.2 10 g glass

192,45 €

8259.3 25 g glass

392,40 €

Op voorraad
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In bestelling
Niet meer verkrijgbaar
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Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS


Algemene informatie

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

Oxidoreductases (catalyse redox reactions)

Transferases (transfer functional groups among substrates)

Hydrolases (cleave bonds via addition of water)

Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

Isomerases (transform chemical isomers)

Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

Oxidoreductases (catalyse redox reactions)

Transferases (transfer functional groups among substrates)

Hydrolases (cleave bonds via addition of water)

Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

Isomerases (transform chemical isomers)

Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Type analysis

Appearancewhite powder
Activity≥45000 FIP U/mg
Water content≤8,0 %
Ash content≤2,0 %