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Proteinase K, 1 g
Transport temp. ambient temp.
WGK 1
CAS No. 39450-01-6
EG-Nr. 254-457-8
Stock solution: 20 mg/ml in water. Storage temperature (stock solution): -20 °C
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
761,10 €/VE Campaign price!
Excl. btw | 1 g Per VE
Bestelnr. 3726.2
Productdetails
Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Foreign activity: RNAse and DNAse not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.
Proteinase K ≥35 U/mg, BioScience Grade, lyophilised
Endopeptidase for the degradation of proteins with very high solubility, specific activity and DNA purity. Extra high purified.
- High solubility
- High specific activity
- Extremely low DNA content
Activity: ≥ 35 U/mg lyophilizate
Specific activity: ≥ 45 U/mg protein
DNA content: ≤ 0.1 pg/mg
Working solution: 50 µg/ml
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS
- Tussentotaal: 0.00
| Bestelnr. | VE | Verp. | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|
| 3726.1 | 100 mg | glass |
213,95 €
171,10 € |
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| 3726.2 | 1 g | glass |
951,40 €
761,10 € |
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
Analysecertificaten
Guarantee analysis
| Appearance | white powder |
| Activity | ≥35 U/mg |
| Specific activity | ≥45 U/mg |
| Solubility in H2O | ≥50 mg/ml |
| Assay DNA | ≤0.1 pg/mg |
| Exonuclease activity | not detectable |
| Endonuclease activity | not detectable |
| Ribonucleases activity | not detectable |