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anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), 0.2 ml

Van  PROGEN
50 μg/ml, monoclonal, affinity purified
Icon_Abweichende-Konditionen Bitte beachten: Abweichende Konditionen / Please note: Different conditions
Verwante producten
VE
Verp.
Antibody, Primary antibodies
Density (D) ~1 g/cm³
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.

Epithelium marker

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
Productdetails

103,10 €/VE 

Excl. btw | 0,2 ml Per VE

Bestelnr. 1NNK.1

Beschikbaar
Vrij van verzendingskosten vanaf 250 €
Klaar voor verzending in 1-2 weken
Vanaf 6 VE 97,94 €/VE
Vanaf 24 VE 92,79 €/VE

Productdetails


This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

  • High epitope affinity
  • Highly specific detection of the target protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence


anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6) 50 μg/ml, monoclonal, affinity purified

Ks 19.2 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma!

Polypeptide Reacting: Mr 40,000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 352-368 (VRADSERQNQEYQRLMD) of the alpha-helical fragment.

Tumors specifically detected: all tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma; thymoma.

Reactivity on cultured cell lines: MCF-7

Host: mouse
Antibody type: monoclonal
Isotype: IgG2b
Clone: Ks19.2 (Z105.6)(also published as BM 19.21, MAK 19.21)
Immunogen: keratin K19 of Mr 40 000; from cultured human MCF-7 cells
UniprotID: P08728 (bovine), P08727 (human), Q63279 (rat)
Synonym: keratin, type I cytoskeletal 19, cytokeratin-19, CK-19, keratin-19, K19, KRT19
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ELISA, ICC/IF, IHC, WB
Reactivity: bovine, human, rabbit, rat
No reactivity: chicken, mouse, woodchuck, xenopus


Toepassing

ELISA: Assay dependent
Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:10-1:50 (1-5 µg/ml)
Immunohistochemistry (IHC) - paraffin: 1:10-1:500 (0.1-5 µg/ml; microwave treatment recommended)
Western Blot (WB): 1:50-1:500 (0.1-1 µg/ml)


1NNK_01
Western blot analysis of human MCF-7 cell lysate with anti-Keratin K19 antibody. Western blot analysis was performed on 10 μg or 5 μg of MCF-7 lysate. Cells were lysed in PBS by homogenization. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6; Cat. No. 690029) was diluted in blocking buffer (antibody concentration 0.1 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
1NNK_02
Human head and neck squamous-cell carcinoma (HNSCC) (courtesy of J. Heß, University)
1NNK_03
Human head and neck squamous-cell carcinoma (HNSCC) (courtesy of J. Heß, University)


Geen medisch hulpmiddel / Geen ivD-product
anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6)
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
1NNK.1 0,2 ml plastic

103,10 €

1NNK.2 1 ml plastic

267,70 €

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Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

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