Technical Data Sheet
Gel Filtration Media ROTI®Dex-25 Medium, 10 g
WGK 1
CAS No. 9041-35-4
Suitable for proteins >5 kDa and oligonucleotides >10 bp.
€84.95/Pack Qty.
excl. VAT. | 10 g per Pack Qty.
Art. No. 21A5.1
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Product details
ROTI®Dex Medium is a spherical, porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin.
In gel filtration, also called size exclusion chromatography, molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules.
In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins.
ROTI®Dex enables a form of group separation for the separation of undesired, low-molecular-weight factors such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.
- Sample preparation in just a few minutes
- Desalting, removal of impurities and buffer exchange in a single step
- No interaction between analyte and medium
- High selectivity, high resolution and high yield (> 95%)
- Also suitable for small sample volumes
- High chemical stability and therefore compatible with many organic and other solvents
An efficient column packing is essential for a clean separation of biomolecules. Please refer to our recommended protocol in the instructions for use.
Gel Filtration Media ROTI®Dex-25 Medium ROTI®Dex
ROTI®Dex-25 medium is excellent for the separation of proteins >5 kDa and nucleic acids >10 bp. Biomolecules are not significantly diluted during purification with ROTI®Dex-25 medium.
Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.
| Matrix | Cross-linked dextran |
| MWCO |
5 kDa 10 bp |
| Particle size |
Dry: 50-150 µm Wet: 85-260 µm |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 21A5.1 | 10 g | plastic |
€84.95 |
|
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| 21A5.2 | 50 g | plastic |
€212.85 |
|
|
| 21A5.3 | 100 g | plastic |
€364.45 |
|
|
| 21A5.4 | 250 g | plastic |
€858.95 |
|
|
| 21A5.5 | 500 g | plastic |
€1,589.95 |
|
|
|
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- Subtotal: 0.00
Downloads / MSDS
General information
Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!
The ROTI®Dex ready-to-use columns at a glance
| Application | Order No. | Matrix | Particle size (wet) | Gel bed volume | Sample volume | MWCO | Pack Qty. |
| Spin | 21C4.1 | ROTI®Dex 25 Medium | 85-260 µm | 0.5 ml | 2-100 µl (50 µl optimal) | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
25 Pcs |
| 21C5.1 | ROTI®Dex 50 Medium | 100-300 µm | 0.5 ml | 2-100 µl (50 µl optimal) | Proteins >25 kDa, Oligonucleotides >20 bp, Nanoparticles >4 nm |
25 Pcs | |
| Grav | 21AX.1 | ROTI®Dex 25 Medium | 85-260 µm | 1.78 ml | 0.15-0.3 ml | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
50 Pcs |
| 21AY.1 | 2.75 ml | 0.5 ml | 50 Pcs | ||||
| 21C0.1 | 4.31 ml | 1 ml | 50 Pcs | ||||
| 21C1.1 | 10.37 ml | 2.5 ml | 25 Pcs | ||||
| 21C2.1 | 17.2 ml | 5 ml | 10 Pcs | ||||
| 21C3.1 | 34.21 ml | 10 ml | 10 Pcs | ||||
| FPLC | 21C6.1 | ROTI®Dex 25 Superfine | 40-110 μm | 1 ml | 0.05-0.3 ml | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
5 Pcs |
| 21C6.2 | 1 ml | 0.05-0.3 ml | 100 Pcs | ||||
| 21C7.1 | 5 ml | 0.1-1.5 ml | 5 Pcs | ||||
| 21C7.2 | 5 ml | 0.1-1.5 ml | 25 Pcs | ||||
| 21C7.3 | 5 ml | 0.1-1.5 ml | 100 Pcs (4x25) |
Certificates of Analysis
Type analysis
| Appearance | white solid |
| Water absorption | 2.15-2.25 ml/g |
| Particle size distribution | |
| 50-150 µm (dry) | ≥80 % |
| Swelling | 4-6 ml/g |