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ROTI®Cell Trypsin/EDTA solution (10x)

CELLPURE® 0.5 % in DPBS, ready-to-use, sterile
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EUH208
i
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Cell dissociation solution
Density (D) 1 g/cm³
Storage temp. -20 °C
Transport temp. cooled

For detachment of adherent cells from tissue and culture surfaces.
Thawing should be done in a +37 °C water bath or overnight at +2 °C to +8 °C. Swirl gently to homogenize.

Trypsin is a protein-cleaving digestive enzyme of the pancreas belonging to the serine proteases, which cleaves lysine and arginine at the C-terminus. Trypsin solutions are used for the separation of cells from tissue or cell monolayers. Thus, they are essential for routine applications in cell culture passaging, for cell culture experiments to obtain an adequate number of cells or for dissociation of primary tissue.
As an alternative to Trypsin, the morecell-protective solution ROTI®Cell Accutase solution can be used, which is free of mammalian components.
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€37.55 /Pack Qty.

€31.85/Pack Qty.  Campaign price!

excl. VAT. | 100 ml per Pack Qty.

Art. No. 1Y19.1

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Product details

Sterile

Trypsin is a protein-cleaving digestive enzyme of the pancreas belonging to the serine proteases, which cleaves lysine and arginine at the C-terminus. Trypsin solutions are used for the separation of cells from tissue or cell monolayers. Thus, they are essential for routine applications in cell culture passaging, for cell culture experiments to obtain an adequate number of cells or for dissociation of primary tissue.
As an alternative to Trypsin, the morecell-protective solution ROTI®Cell Accutase solution can be used, which is free of mammalian components.



ROTI®Cell Trypsin/EDTA solution (10x) CELLPURE® 0.5 % in DPBS, ready-to-use, sterile

This trypsin solution contains EDTA (0.22%). EDTA is a chelating agent and enhances the ability of trypsin to detach adherent cells. EDTA binds calcium and magnesium, which additionally weakens cell contacts. This favors the hydrolysis of specific peptide bonds by trypsin.


The required trypsin concentration depends primarily on the cell type and the age of the cell culture. For orientation, you will find a complete protocol in the technical info brochure.


After cell dissociation, trypsin should be inhibited. This can be done either by using FCS or trypsin inhibitors.



Technical Information
End concentration 1x 
Enzyme Trypsin from porcine pancreas 
Alternative Name Cell dissociation reagents 
Use Cell dissociation 
Instructions for use, addition Preparation of aliquots recommended to avoid repeated freeze-thaw cycles. 
ROTI®Cell Trypsin/EDTA solution (10x)
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The following analysis certificates have been found:

Type analysis

pH value7.0-7.6
Osmolality280-350 mOsm/kg
Endotoxins≤10.0 EU/ml
Sterilitycomplies