Technical Data Sheet
5-Bromo-4-chloro-3-indolyl phosphate disodium salt, 500 mg
Molar mass (M) 397,46 g/mol
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
CAS No. 102185-33-1
Protect from light and humidity.
Substrate for alkaline phosphatase, in particular in immunoblot procedures.
€161.25/Pack Qty.
excl. VAT. | 500 mg per Pack Qty.
Art. No. A155.2
Product details
5-Bromo-4-chloro-3-indolyl phosphate disodium salt ≥99 %
In combination with NBT ideal for colorimetric detection of alkaline phosphatase activity, in particular in immunoblot procedures.
Application | Western, in situ, Histochemistry |
Enzyme | Alkaline phosphatase |
Detection | Colour (brown-violet) |
Signal product | Precipitate (Blot) |
Use | Western, in situ, histochimie |
- Subtotal: 0.00
Art. No. | Pack Qty. | Pack. | Price | Quantity | |
---|---|---|---|---|---|
A155.1 | 100 mg | glass |
€37.55 |
|
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A155.2 | 500 mg | glass |
€161.25 |
|
|
A155.3 | 1 g | glass |
€289.20 |
|
|
A155.4 | 5 g | glass |
€923.45 |
|
|
In stock
Available
In procurement
No longer available
Delivery date currently unknown
|
- Subtotal: 0.00
Downloads / MSDS
General information
Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.
Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).
Always prepare freshly!
Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.
Reference: Bos E.S. et al. (1981) J. Immunoassay 2, (3/4), 187.
Certificates of Analysis
Type analysis
Appearance | almost white powder |
Assay (titr.) | ≥99.0 % |
Water (KF) | 5.8-7.8 % |