Technical Data Sheet
ROTI®Spin MAXI 10 kDa
€118.25/Pack Qty.
excl. VAT. | 8 unit(s) per Pack Qty.
Art. No. 25TL.1
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Product details
For purification and concentration of biomolecules and for purification of solutions.
- ROTI®Spin MINI: 1.5 ml sample tube for up to 500 µl sample
- ROTI®Spin MIDI: 15 ml sample tube for up to 5 ml sample
- ROTI®Spin MAXI: 50 ml sample tube for up to 15 ml sample
Centrifugal filters for ultrafiltration of biomolecules. The membranes made of highly chemical-resistant polyethersulfone have been tightly sealed to prevent leakage and loss of samples. Furthermore, the membranes underwent special treatment minimising the binding of biomolecules, in order to optimise sample recovery rates.
ROTI®Spin Centrifugal filters can be applied to a variety of applications and can be used to replace tedious and labour-intensive methods like gel filtration, gel purification or precipitation. Application is time-saving and very simple - only few centrifugal steps are necessary to concentrate and recover your sample. During this, the sample remains untouched in the upper reservoir, and cross-contamination is efficiently prevented even when large numbers of samples are handled.
Desalting or buffer exchange following restriction digest, PCR or such; separation of free nucleotides and polymerised DNA; removal of contaminating compounds (like DNA) from stock solutions (e.g. PCR-buffers); concentration of DNA or proteins; separation of proteins of different size; sample preparation prior to HPLC.
| Overview for selecting the appropriate pore size | ||
| MWCO | Molecule size | DNA size |
| 10 kDa | 30 – 90 kDa | 50 – 200 bp |
| 30 kDa | 90 – 180 kDa | 200 – 500 bp |
| 50 kDa | 150 – 300 kDa | 500 bp – 1 kb |
| 100 kDa | >300 kDa | >1 kb |
ROTI®Spin MAXI 10 kDa
Sample volumes:
Initial volume: max. 15 ml
Final volume: 200 µl
MWCO:
Centrifugation units with membranes in 4 different molecular weight cut-offs (MWCO) are available for specific applications. Select the appropriate MWCO according to the following rule:
Purification of proteins/peptides: MWCO ≤1/3 of the sample molecule size.
Purification of nucleic acids: MWCO ≤1/2 of the sample molecule size.
- Suitable for nucleic acids, proteins/peptides, other biomolecules >20 kDa and very small bioparticles
- Replaces standard applications such as precipitation, dialysis, gel filtration, gel purification, column chromatography, gradient centrifugation, etc.
- Gentle: no shearing of DNA up to 100 kb, proteins remain native and enzymatically active
- Can be used without restriction for labelled samples (isotope, fluorescence, chromogenic labelling)
- Very high recovery rates: with the right MWCO, the yield is >90%"
| Type | Centrifugation filter |
| MWCO | 10 kDa |
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| Art. No. | Type | Pack Qty. | Price | Quantity | |
|---|---|---|---|---|---|
| 25TL.1 | Centrifugation filter | 8 unit(s) |
€118.25 |
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