Technical Data Sheet
ROTI®Free Stripping Buffer 2.0, 1 l
Boiling point (bp) ~100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 8 III
WGK 1
UN-Nr. 1760
Premixed buffer for gentle stripping of Western-Blot membranes after Chemoluminescence detection.
€138.70/Pack Qty.
excl. VAT. | 1 l per Pack Qty.
Art. No. 3319.2
Fits to / Accessories
Product details
ROTI®Free Stripping Buffer 2.0 ready-to-use
Stripping buffer for efficient removal of primary and secondary antibodies, as well as chemoluminescence staining, from Western blot membranes. Due to the low pH of the buffer, the antibody conformation is altered, therefore gently removing all molecules bound to the epitopes (Legocki et. al. (1981) Analyt. Biochem. 111:385-92). Robust formulation without β-mercaptoethanol or DTT for use at room temperature. Suitable for nitrocellulose and PVDF membranes.
- Effective
- Gentle
- Odour-free
- Simple application w/o heating
- Cost-efficient
Following rinsing steps in PBST- or TBST buffer, the membrane is incubated in ROTI®Free Stripping-Puffer 2.0 for 15-60 mins. at room temperature. After washing it again, the membrane may be blocked and probed anew. The particular time needed for complete stripping depends on the respective antibody/epitope pairing.
- Subtotal: 0.00
Art. No. | Pack Qty. | Pack. | Price | Quantity | |
---|---|---|---|---|---|
3319.1 | 250 ml | glass |
€53.65 |
|
|
3319.2 | 1 l | glass |
€138.70 |
|
|
In stock
Available
In procurement
No longer available
Delivery date currently unknown
|
- Subtotal: 0.00
Downloads / MSDS
Certificates of Analysis
Notes on the product
Remark: Contains SDS. Can be redissolved by heating (max. 45 °C). |