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ROTI®Load DNAstain 2 SYBR® Green, 1.8 ml, 1 x 1.8 ml
Ready-to-use products and reagents
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
Contains: Tris, EDTA, xylencyanol, tartrazine, SYBR® Green.
1.8 ml are sufficient for gel run and staining of approx. 1,800 lanes (5 µl sample + 1 µl loading buffer).
53,65 €/VE
Excl. btw | 1,8 ml Per VE
Bestelnr. 1CN6.1
Productdetails
ROTI®Load DNAstain 2 SYBR® Green 6x conc., ready-to-use
| Activeringsmax. (DNA-gebonden) |
~320 nm 470-500 nm |
| Aantal sporen | ca. 1000/ml |
| Emissiemax. (DNA-gebonden) | 525 nm |
| Kleurstof | Xylene cyanole, tartrazine, SYBR® Green |
| Incl. DNA-kleuring | ja |
| Sensitiviteit | 0,01 ng/band |
| Toepassing | Simultaneous gel staining, medium fragments |
- Tussentotaal: 0.00
| Bestelnr. | VE | Verp. | Verpakking | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|---|
| 1CN6.1 | 1,8 ml | plastic | 1 x 1.8 ml |
53,65 € |
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| 1CN6.2 | 9 ml | plastic | 5 x 1.8 ml |
166,65 € |
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Op voorraad
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Leveringsdatum onbekend
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
Care should be taken to ensure that the dyes contained in the gel stop before smallest relevant DNA bands. This ensures that the buffer can be stopped in time. However, the selected dyes may overlay the bands shown. In this case, select a loading buffer that does not contain any unwanted dyes.
Bromophenol blue and xylene cyanol can be used as colour markers in all standard gels. If a relevant band is overlaid by one of these colour markers, choose a gel loading buffer containing Orange G. If small fragments are to be assayed, the gel loading buffer should contain Orange G in order to mark the run. If relevant bands are overlaid, however, a choice can be made between bromophenol blue or xylene cyanol depending on the size of the bands. A loading buffer without xylene cyanol is usually used for assaying large fragments.
Glycerine is the standard reagent for increasing the density of samples.
Ficoll® 400 produces particularly well-defined bands.
Summary Staining of Nucleic Acid in Gels
| Product | Sensitivity | Description | Excitation |
| Ethidium bromide solution | 0.5-5 ng/band | Standard fluorescent staining | 254-330 nm, 500 nm UV- and blue light |
| SYBR® Green DNA dye | 0.01 ng/band | Non-toxic, green fluorescent staining | 254 nm and 495 nm UV- and blue light |
| ROTI®GelStain | 0.3 ng/band | Non-toxic, green fluorescent staining | 302 nm and 490 nm UV- and blue light |
| ROTI®GelStain Red | 0.3 ng/band | Non-toxic, red fluorescent staining | 310 nm, 540 nm UV- and blue light |
| ROTI®Methylene blue staining concentrate | 10 ng/band | Reversible, non-toxic blue staining | White light |
| ROTI®Black N | <0.1 ng/band | Silver staining of DNA polyacrylic amide gels | White light |
Analysecertificaten
Notes on the product
| Appearance | green solution |
| Each batch is functionally tested for its suitability in electrophoresis. | |