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ROTI®Blot 1

10x conc., for electrophoresis
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Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
Warning
H290-H315-H319
i may be corrosive to metals, causes skin irritation, causes serious eye irritation
P280 P302+P352 P305+P351+P338
i wear protective gloves/eye protection, IF ON SKIN: Wash with plenty of water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Packaging
Blotting buffers
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 1

Discontinuous transfer buffer for semi dry blotting.
Kit of two solutions. Both solutions may be bought separately.

Discontinuous buffer system for optimum transfer in the semi-dry blotter.
Product details

€107.40/Pack Qty. 

excl. VAT. | 1 kit per Pack Qty.

Art. No. L509.1

Available at short notice
from 6 Pack Qty. €102.03/Pack Qty.
from 24 Pack Qty. €96.66/Pack Qty.
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Product details


Discontinuous buffer system for optimum transfer in the semi-dry blotter.

  • Optimised for semi dry blotting
  • Superior blotting results for peptides and proteins of every size
  • Special formulation with reduced acid formation - for optimal electrode protection

In the semi-dry blotting system, both a continuous buffer system (identical buffer at anode and cathode) and a discontinuous buffer system (different buffer at anode and cathode) can be used. The transfer performance of discontinuous systems is, however, generally higher, since the two buffers are designed separately according to the needs of the two electrodes.

The discontinuous buffer system ROTI®Blot 1 consists of two different buffers, the anode buffer A (pH 7.8±0.1) and the cathode buffer K (pH 8.5±0.1). ROTI®Blot 1 shows a much better transfer efficiency than a tris-glycine buffer and can be used for proteins and peptides of any length.


Directions for use

The blot stack is built up by soaking the upper blotting papers with cathode buffer and the lower blotting papers with anode buffer. With slightly longer transfer times, proteins >100 kDa can also be completely transferred with ROTI®Blot 1.

A detailed instruction manual is enclosed with the product.



ROTI®Blot 1 10x conc., for electrophoresis
The kit contains

Anode buffer ROTI®Blot A, 1 l (10 x concentration) (Art. No. L510)
Cathode buffer ROTI®Blot K, 1 l (10 x concentration) (Art. No. L511)
Both solutions may be bought separately.



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