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ROTIPHORESE®5x TBE Buffer, 1 l, glass

ROTIPHORESE® 5x conc.
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Pack Qty.
Pack.
TBE buffer
Density (D) 1,031 g/cm³
Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 1

TBE-buffer for preparing sequencing gels and as running buffer in electrophoresis.
Concentrated TBE tends to precipitate salts during storage. Mix before use!

The TBE buffer is optimally suited as a gel and running buffer system.
Product details

€30.65/Pack Qty. 

excl. VAT. | 1 l per Pack Qty.

Art. No. 1L9K.1

In production
from 6 Pack Qty. €29.12/Pack Qty.
from 24 Pack Qty. €27.58/Pack Qty.

Product details


The TBE buffer is optimally suited as a gel and running buffer system.


Mechanism

TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.
ROTIPHORESE®TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels.



ROTIPHORESE®5x TBE Buffer ROTIPHORESE® 5x conc.

ROTIPHORESE®5x TBE buffer contains 0,5 M Tris-Borate pH 8,3 and 10 mM EDTA in distilled, deionised water; pH 8,3.

Suitable for manufacturing sequencing gels with ROTIPHORESE®Gel 40 and as a running buffer.


Directions for use

ROTIPHORESE®TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA.


Mechanism

TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.



ROTIPHORESE®5x TBE Buffer
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Art. No. Pack Qty. Pack. Price Quantity
1L9K.1 1 l glass

€30.65

1L9K.2 2.5 l glass

€66.15

1L9K.3 5 l plastic

€107.00

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Composition

Appearanceclear, colorless solution
0.5 M TRIS borate (pH 8.3), 10 mM EDTA., distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C).