Technical Data Sheet
ROTIPHORESE®5x TBE Buffer, 1 l, glass
Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 1
Concentrated TBE tends to precipitate salts during storage. Mix before use!
€30.65/Pack Qty.
excl. VAT. | 1 l per Pack Qty.
Art. No. 1L9K.1
Fits to / Accessories
Product details
The TBE buffer is optimally suited as a gel and running buffer system.
TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.
ROTIPHORESE®TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels.
ROTIPHORESE®5x TBE Buffer ROTIPHORESE® 5x conc.
ROTIPHORESE®5x TBE buffer contains 0,5 M Tris-Borate pH 8,3 and 10 mM EDTA in distilled, deionised water; pH 8,3.
Suitable for manufacturing sequencing gels with ROTIPHORESE®Gel 40 and as a running buffer.
ROTIPHORESE®TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA.
TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.
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Art. No. | Pack Qty. | Pack. | Price | Quantity | |
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1L9K.1 | 1 l | glass |
€30.65 |
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1L9K.2 | 2.5 l | glass |
€66.15 |
|
|
1L9K.3 | 5 l | plastic |
€107.00 |
|
|
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Downloads / MSDS
Certificates of Analysis
Composition
Appearance | clear, colorless solution |
0.5 M TRIS borate (pH 8.3), 10 mM EDTA., distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C). |