Jump to content Jump to navigation menu

ROTIPHORESE®Gel B, 250 ml

ROTIPHORESE® 2 %, gas-stabilized
Icon_EVE-EUD Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Danger
H340-H350-H373
i may cause genetic defects, may cause cancer, may cause damage to organs (peripheral nervous system) through prolonged or repeated exposure (if swallowed)
P260 P280 P308+P313
i do not breathe gas/mist/vapours/spray, wear protective gloves/eye protection, IF exposed or concerned: Get medical advice/attention
Pack Qty.
Pack.
Gel solution, PAGE gel solutions
Density (D) ~1 g/cm³
Boiling point (bp) 100 °C
Melting point (mp) ~0 °C
Storage temp. +4 °C
WGK 3

Aqueous bisacrylamide stock solution for preparation of protein gels in combination with ROTIPHORESE®Gel A or Gel A-40.
Gels with up to 25 % total acrylamide concentration can be produced.

Ready-to-use, gas-stabilized bisacrylamide ready-to-use solution. The ROTIPHORESE® gel solution is very easy to use and enables reproducible and high-resolution gel electrophoresis.
Product details

€25.30/Pack Qty. 

excl. VAT. | 250 ml per Pack Qty.

Art. No. 3039.2

In stock
Delivery fast, simple and reliable!
from 6 Pack Qty. €24.04/Pack Qty.
from 24 Pack Qty. €22.77/Pack Qty.

Product details


Ready-to-use, gas-stabilized bisacrylamide ready-to-use solution. The ROTIPHORESE® gel solution is very easy to use and enables reproducible and high-resolution gel electrophoresis.


ROTIPHORESE® gel solutions can be used for protein and nucleic acid separation in PAGE gels. The high-purity solutions can be combined with any buffer system. During sequencing, they facilitate the preparation of sequencing gels of consistent quality; our fluorescence-free gel solutions are recommended for the use of automatic sequencers.
The ROTIPHORESE® acrylamide and bisacrylamide solutions from Carl ROTH are produced only from acrylic acid-free substances and are strictly controlled in acrylamide content and pH. Oxidation products are removed from the solution by a selective absorption process. Their new formation and the polymerization of the solution are prevented by stabilizing the solution with inert gas.
In order to ensure a high consistency of our ROTIPHORESE® solutions, each batch is subjected to an electrophoretic test.


Mechanism

PAGE solutions are always a mixture of acrylamide and bisacrylamide. Acrylamide forms linear polymers, whereas bisacrylamide crosslinks these linear polymers forming pores of a special size. The pore size may be altered by varying the total gel concentration (% T) and the percentage of the crosslink (% C).
With the ROTIPHORESE® stock solutions the desired T/C rate can be produced. You need an acrylamide stock solution (Gel A or Gel A-40) and a bisacrylamide stock solution (Gel B).


Acrylamide and Bisacrylamide Stock Solutions


PAGE solutions are always a mixture of acrylamide and bisacrylamide. Acrylamide forms linear polymers, whereas bisacrylamide crosslinks these linear polymers forming pores of a special size. The pore size may be altered by varying the total gel concentration (% T) and the percentage of the crosslink (% C).
With the ROTIPHORESE® stock solutions the desired T/C rate can be produced. You need an acrylamide stock solution (Gel A or Gel A-40) and a bisacrylamide stock solution (Gel B).

For detailed instructions regarding gel preparation with ROTIPHORESE® Gel A, A-40 and B please see user manual and technical information brochure “PAGE Instructions”.



ROTIPHORESE®Gel B ROTIPHORESE® 2 %, gas-stabilized
Directions for use

Ready-to-use, stabilised 2 % bisacrylamide stock solution. In combination with ROTIPHORESE®Gel A (30 % acrylamide stock solution, Art. No. 3037) or ROTIPHORESE®Gel A-40 (40 % acrylamide stock solution, Art. No. 7748) gels with up to 25 % total acrylamide concentration can be produced.


For detailed instructions regarding gel preparation with ROTIPHORESE®Gel A, A-40 and B please see our Technical Information Brochure “PAGE Instructions”.



ROTIPHORESE®Gel B
Selected quantity:   0
  1. Subtotal:  0.00
Art. No. Pack Qty. Pack. Price Quantity
3039.1 1 l glass

€61.20

3039.2 250 ml glass

€25.30

In stock
Available
In procurement
No longer available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS


General information

Safe
Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE® ready-to-use solution.

Superior quality
ROTIPHORESE® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE®-solutions, each batch undergoes electrophoretic testing.


The right choice for each and every application
The large variety of different ROTIPHORESE® solutions provides you with the right product for each and every application. ROTIPHORESE® gel solution may be used both in protein and nucleic acid electrophoresis. ROTIPHORESE® ready-to-use solutions for manual sequencing facilitate the preparation of sequencing gels of a constant high quality - also suitable for automatic sequencers.


ROTIPHORESE®Gel 30 and Gel 40 are ready-to-use, gas-stabilised acrylamide solutions with different concentrations and with different ratios of acrylamide to bisacrylamide. ROTIPHORESE® ready-to-use solutions can be used directly.


Recommendation for Use of ROTIPHORESE® Ready-Made Gel Solutions

Analysis of Gel solution Art. No.
Proteins/peptides >15 kDa, DNA fragments Gel 30 (37,5:1) 3029.1
Gel 40 (37,5:1) T802.1
Proteins/peptides <30 kDa and DNA oligonucleotides Gel 40 (29:1) A515.1
DNA sequences (manual sequencing) ROTIPHORESE®DNA sequencing system A431.1
Gel 40 (19:1) 3030.1
DNA sequences (short-run automat. sequencing) NF solution 40 % (19:1) A516.1
DNA sequences (long-run automat. sequencing) NF solution 30 % (29:1) A124.1
NF solution 40 % (29:1) A121.1

Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Notes on the product

Filled under protective gas., Each batch is functionally tested for its suitability in electrophoresis.