ROTI®-Mark WESTERN PLUS, 500 µl, 2 x 250 µl
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
Number of protein bands: 14
- 10 IgG binding
- 4 prestained (tricolour)
Number of lanes/ml: approx. 200 (mini gel)
For direct application to the gel.
For the most common type of primary antibodies are IgG type antibodies the ROTI®Mark WESTERN PLUS ladder can be used in almost any situation of Western Blot. Appropriate detection methods are fluorescence, chemoluminescence, colour or other systems.
€482.70/Pack Qty.
excl. VAT. | 500 µl per Pack Qty.
Art. No. 2245.2
Fits to / Accessories
Product details
ROTI®-Mark WESTERN PLUS ready-to-use, pre-dyed
The ROTI®Mark WESTERN PLUS protein ladder covers a wide range of molecular weights from 15 to 200 kDa. It consists of 10 IgG binding proteins with two in their mass enhanced reference bands (30 kDa and 80 kDa).
For the most common type of primary antibodies are IgG type antibodies the ROTI®Mark WESTERN PLUS ladder can be used in almost any situation of Western Blot. Appropriate detection methods are fluorescence, chemoluminescence, colour or other systems.
For controlling the gel run during SDS-PAGE and for reliable evaluation of transfer efficiency during Western Blot the ladder additionally contains four prestained proteins: 10 kDa (blue), 25 kDa (green), 45 kDa (blue) und 70 kDa (red).
Fig.: Marker after detection on X-ray film (left), after Western blot on PVDF membrane (right)
Number of fragments | 14 |
Number of protein bands |
14: 10 lgG binding 4 prestained (tricolored) |
Amount of traces | ~200/ml |
Colour | prestained |
Size range | 10-200 kDa |
Detection in western blot | detection by anti-His possible |
Quantification | not recommended |
Dispatch | ready-to-use |
Reinforced bands at | 30 kD, 80 kD |
- Subtotal: 0.00
Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
---|---|---|---|---|---|---|
2245.1 | 250 µl | plastic | 1 x 250 µl |
€246.20 |
|
|
2245.2 | 500 µl | plastic | 2 x 250 µl |
€482.70 |
|
|
In stock
In procurement
No longer available
Delivery date currently unknown
|
- Subtotal: 0.00
Downloads / MSDS
General information
Marker | ALL BLUE | BI-PINK | TRICOLOR | TRICOLOR XTRA |
Art. No. | 2242 (fig. 1) | 8269 (fig. 2) | 8271 (fig. 3) | 2244 (fig. 4) |
Size Range (kD) | 10-240 | 10-175 | 10-245 | 10-310 |
Number of Fragments | 12 | 11 | 12 | 12 |
Reinforced band at (kD) | 25, 72 | - | - | - |
Quantitation | not recomm. | not recomm. | not recomm. | not recomm. |
Staining | blue prestained | pink prestained | blue prestained | blue prestained |
Additional reference bands | - | 10 kD, 40 kD, 90 kD blue | 25 kD green, 75 kD red | 25 kD green, 75 and 310 kD red |
Detection in Western-Blot | no detection | no detection | no detection | no detection |
Number of Lanes in minigel | 200 / ml | 200 / ml | 200 / ml | 200 / ml |
Delivery | ready-to-use | ready-to-use | ready-to-use | ready-to-use |