Naar de inhoud springen Naar het navigatiemenu springen

ROTI®Load 2

4x conc., unreducing
Icon_ready-to-use Ready-to-use products and reagents
VE
Verp.
Gel loading buffer, Laemmli buffer
Density (D) 1,158 g/cm³
Boiling point (bp) 100 °C
Melting point (mp) 0 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 2

Protein gel loading buffer, modified formulation acc. to Laemmli. For protein complexes, secondary structures, and for native gels.
Contains SDS (approx. 8 % w/v), glycerol (approx. 40 % v/v) and bromophenol blue (approx. 0,015 % w/v). Phosphate buffered.

ROTI®Load 1 (reducing) as well as ROTI®Load 2 and 3 (non-reducing) represent special gel loading buffers for protein gel electrophoresis. They have been designed to protect your proteins during sample preparation and to stabilise sensitive peptide bonds. Protein degradation is efficiently prevented by those buffer systems. ROTI®Load are 4x concentrated ready-to-use solutions, which may be used directly for sample loading.
Productdetails
Type analysis

16,70 €/VE 

Excl. btw | 10 ml Per VE

Bestelnr. K930.1

Binnenkort beschikbaar
Vrij van verzendingskosten vanaf 125 €
Vanaf 6 VE 15,86 €/VE
Vanaf 24 VE 15,03 €/VE
Carl Roth abonnementservice

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:
1

Put all products for your subscription in the desired quantity in the basket.

2

In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.

3

Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Productdetails


ROTI®Load 1 (reducing) as well as ROTI®Load 2 and 3 (non-reducing) represent special gel loading buffers for protein gel electrophoresis. They have been designed to protect your proteins during sample preparation and to stabilise sensitive peptide bonds. Protein degradation is efficiently prevented by those buffer systems. ROTI®Load are 4x concentrated ready-to-use solutions, which may be used directly for sample loading.



ROTI®Load 2 4x conc., unreducing

Well tested and proven sample buffer for protein gel electrophoresis that has been modified and further developed based on the original formulation acc. to Laemmli (Nature 227, 1970). For depiction of protein complexes, secondary structures, and for native gels.

  • Formulation acc. to Laemmli (modified)
  • Stabilises peptide bands
  • For protein complexes and secondary structures
  • For native gels

Gebruiksinstructies

ROTI®Load protein gel loading buffers are usually used as 1x concentrated solution. ROTI®Load can either be added to the prepared sample or the precipitated proteins are dissolved directly into ROTI®Load. Optionally, heat the ROTI®Load mixed sample to 95 °C for 5 minutes to break hydrogen bonds and stretch the peptide chains.


ROTI®Load 2 prevents degradation of proteins while heating during sample preparation, additionally keeping the sample pH value constant during gel electrophoresis. Being a phosphate-buffered ready-to-use solution, ROTI®Load 2 already contains all necessary denaturing reagents, bromophenol blue as dye and glycerol as density increasing reagent. ROTI®Load 2 may be supplemented with 20 µl/ml DTT solution (1 M), in order to gain a loading buffer using DTT as reducing reagent rather than β-Mercaptoethanol.



ROTI®Load 2
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
K930.1 10 ml glass 16,70 €
Op voorraad
Binnenkort beschikbaar
Niet beschikbaar
Leveringsdatum onbekend op dit moment
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads


Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Type analysis

Appearancedark blue, clear solution  
pH value (1x sol.)6,7-7,2  
Remark: Contains SDS. Can be redissolved by heating (max. 45 °C).

0 Beoordelingen

0.0 van 5 sterren