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ROTIPHORESE®NF-Acrylamide/Bis-solution 40 (19:1), 250 ml, glass

ROTIPHORESE® 40 %, ready-to-use, gas-stabilized, fluorescence-free
Icon_ready-to-use Ready-to-use products and reagents
Icon_EVE-EUD Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
Examples of effect: In smaller quantities, lead to immediate serious damage to health or death. Safety: Do not inhale, touch or swallow. Wear work safety equipment. Call a poison control centre or doctor immediately. Recovery position.
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Danger
H301-H315-H317-H319-H332-H340-H350-H361f-H372
i toxic if swallowed, causes skin irritation, may cause an allergic skin reaction, causes serious eye irritation, harmful if inhaled, may cause genetic defects, may cause cancer, suspected of damaging fertility (if swallowed), causes damage to organs through prolonged or repeated exposure
P201 P280 P301+P310 P302+P352 P305+P351+P338 P308+P313
i obtain special instructions before use, wear protective gloves/protective clothing/eye protection/face protection, IF SWALLOWED: Immediately call a POISON CENTER/doctor, IF ON SKIN: Wash with plenty of soap and water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, IF exposed or concerned: Get medical advice/attention
Related products
Pack Qty.
Pack.
Gel solution, PAGE gel solutions
Density (D) 1,03 g/cm³
Boiling point (bp) ~100 °C
Storage temp. +4 °C
ADR 6.1 III
WGK 3
UN-Nr. 3426

Aqueous acrylamide and bisacrylamide stock solution at a ratio of 19:1 for PAGE and automatic DNA sequencers.
Optimal for DNA gels for separation of small fragments, manual sequencing and automatic sequencers. Cross linking 5.

Ready-to-use, gas-stabilized ready-to-use acrylamide solutions with different concentrations and ratios of acrylamide to bisacrylamide. The ROTIPHORESE® gel solutions are very easy to use and enable reproducible and high-resolution gel electrophoresis.
Product details
€74.75 /Pack Qty.

€56.00/Pack Qty.  Campaign price!

Purchase before 31.05.2024 23:59 to benefit from this special price!

excl. VAT. | 250 ml per Pack Qty.

Art. No. A516.1

In stock
Delivery fast, simple and reliable!

Product details


Ready-to-use, gas-stabilized ready-to-use acrylamide solutions with different concentrations and ratios of acrylamide to bisacrylamide. The ROTIPHORESE® gel solutions are very easy to use and enable reproducible and high-resolution gel electrophoresis.


Directions for use

ROTIPHORESE® gel solutions can be used for protein and nucleic acid separation in PAGE gels. The high-purity solutions can be combined with any buffer system. During sequencing, they facilitate the preparation of sequencing gels of consistent quality; our fluorescence-free gel solutions are recommended for the use of automatic sequencers.


Please note

The ROTIPHORESE®acrylamide and bisacrylamide solutions from Carl ROTH are produced only from acrylic acid-free substances and are strictly controlled in acrylamide content and pH. Oxidation products are removed from the solution by a selective absorption process. Their new formation and the polymerization of the solution are prevented by stabilizing the solution with inert gas.
In order to ensure a high lot-to-lot consistency of our ROTIPHORESE® solutions, each batch is subjected to an electrophoretic test. Fluorescence-free gel solutions are tested for suitability for automatic sequencers.


NF acrylamide solutions are very well suited for application in fluorescence-labelled automated sequencing, e.g. using LI-COR or ABI slab gel sequencers.


ROTIPHORESE®NF ready-to-use solutions for automated sequencing are special fluorescence-free acrylamide/bisacrylamide mixtures of highest quality as required for automatic sequencing. Made from 4x cristallised acrylamide.


• For preparing 4-20 % sequencing gels
• No tedious dissolving of urea
• Prevents acrylamide dusts
• Simple handling



ROTIPHORESE®NF-Acrylamide/Bis-solution 40 (19:1) ROTIPHORESE® 40 %, ready-to-use, gas-stabilized, fluorescence-free

Cross linking 5.


ROTIPHORESE®NF ready-to-use solutions are made from 4x crystallized acrylamide and are special fluorescence-free acrylamide/bisacrylamide mixtures of the highest quality, as required for automatic sequencing.



ROTIPHORESE®NF-Acrylamide/Bis-solution 40 (19:1)
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Art. No. Pack Qty. Pack. Price Quantity
A516.1 250 ml glass
€74.75

€56.00

A516.2 250 ml plastic
€80.55

€80.55

A516.3 100 ml plastic
€39.70

€39.70

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Available
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No longer available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS


General information

Safe
Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE® ready-to-use solution.

Superior quality
ROTIPHORESE® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE®-solutions, each batch undergoes electrophoretic testing.


The right choice for each and every application
The large variety of different ROTIPHORESE® solutions provides you with the right product for each and every application. ROTIPHORESE® gel solution may be used both in protein and nucleic acid electrophoresis. ROTIPHORESE® ready-to-use solutions for manual sequencing facilitate the preparation of sequencing gels of a constant high quality - also suitable for automatic sequencers.


ROTIPHORESE®Gel 30 and Gel 40 are ready-to-use, gas-stabilised acrylamide solutions with different concentrations and with different ratios of acrylamide to bisacrylamide. ROTIPHORESE® ready-to-use solutions can be used directly.


Recommendation for Use of ROTIPHORESE® Ready-Made Gel Solutions

Analysis of Gel solution Art. No.
Proteins/peptides >15 kDa, DNA fragments Gel 30 (37,5:1) 3029.1
Gel 40 (37,5:1) T802.1
Proteins/peptides <30 kDa and DNA oligonucleotides Gel 40 (29:1) A515.1
DNA sequences (manual sequencing) ROTIPHORESE®DNA sequencing system A431.1
Gel 40 (19:1) 3030.1
DNA sequences (short-run automat. sequencing) NF solution 40 % (19:1) A516.1
DNA sequences (long-run automat. sequencing) NF solution 30 % (29:1) A124.1
NF solution 40 % (29:1) A121.1

Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Notes on the product

Filled under protective gas., Tested for automatic sequencers., Each batch is functionally tested for its suitability in electrophoresis., Note: Product may crystallise. It can be liquefied by heating in a water bath to max. 40 °C.