Technical Data Sheet
Ethidium bromide, 5 g
Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
Molar mass (M) 394,33 g/mol
Density (D) 1,01 g/cm³
Flash point (flp) >100 °C
Melting point (mp) 264 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 6.1 I
WGK 1
CAS No. 1239-45-8
EG-Nr. 214-984-6
UN-Nr. 2811
For DNA-staining.
€131.70/Pack Qty.
excl. VAT. | 5 g per Pack Qty.
Art. No. 7870.2
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Ethidium bromide is a strong mutagenic and toxic substance. For your own safety, always wear gloves and respiratory protection to prevent contamination when working with this substance. After use, dispose of solutions correctly. They may not be discarded into the environment.
The following procedure is recommended (acc. to INSERM, Dossier prevention, Number 2, 2nd Ed., September 88):
1. Collect and dispose of as hazardous waste.
2. Break down EtBr with potassium permanganate and HCI (P. Quillardet, M. Hofnung, Trends Genet. 4,89 [1988]).
3. Break down EtBr with hypophosphoric acid and sodium nitrite (Lunn and Sansone; Analyt. Biochem. 162, 453-458 [1987]).
4. Absorption of EtBr with Amberlite® XAD-16 (Lunn and Sansone) or with activated carbon (Bensaude, O. Method, Trends Genet. 4, 89-90 [1988]) from the solution.
We recomment disposure by SEKUROKA®Decon-Bags (Art. No. T856)
Ethidium bromide ≥98 %
Fluorescent dye with specific DNA binding capacity. Intercalates between base pair stacks of dsDNA, causing weaker staining of ssDNA or RNA. For staining of DNA/RNA in gels and for counterstaining of nuclei in histological and cytological analyses.
Excitation maximum: 518 nm
Emission maximum: 605 nm
| Dyeing | dead cells red fluorescent |
| Max. excitation (unbound) | 518 nm |
| Emissions maximum | 605 nm |
| Use | vitality test |
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| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 7870.1 | 1 g | glass |
€33.25 |
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| 7870.2 | 5 g | glass |
€131.70 |
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Available (note delivery time)
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
For brilliant results in the laboratory and research
Discover our extensive range of dyes for demanding applications in science and industry. Whether for microscopy, histology, biochemistry or industrial applications, you will find the right dye here.
The content of colourants is not specified for many products for various reasons:
1. complex composition: colourants are often mixtures of several components that may be present in different ratios. These compositions can vary, which makes it difficult to state an exact content.
2. application focus: In many applications, especially in the textile and food industries, the exact concentration of a dye is less relevant than its colouring power or its ability to achieve a certain colour. Here, the colour strength or the ability to impart colour is the most important criterion.
3. standardisation: Many dyes are traded according to certain standards (e.g. C.I. numbers) which guarantee a certain quality and colouring. These standards often make an exact specification of content superfluous, as users know that the colourant has the desired properties.
4. manufacturing processes: The manufacturing process of colourants can lead to natural variations in content. Instead of specifying an exact content, quality control is often carried out to ensure that the colourant is within an acceptable range.
5. Regulatory aspects: In some cases, regulatory requirements may not require the manufacturer to declare the exact content as long as the colourant is safe and effective for the intended purpose.
6. cost reasons: An exact determination of the content often requires complex analytical procedures that could increase production costs. Therefore, some manufacturers do not provide this additional information in order to save costs.
In summary, it can be said that the content of colourants is often not specified because other quality criteria are in the foreground and the exact concentration is not decisive for many applications.
Summary Staining of Nucleic Acid in Gels
| Product | Sensitivity | Description | Excitation |
| Ethidium bromide solution | 0.5-5 ng/band | Standard fluorescent staining | 254-330 nm, 500 nm UV- and blue light |
| SYBR® Green DNA dye | 0.01 ng/band | Non-toxic, green fluorescent staining | 254 nm and 495 nm UV- and blue light |
| ROTI®GelStain | 0.3 ng/band | Non-toxic, green fluorescent staining | 302 nm and 490 nm UV- and blue light |
| ROTI®GelStain Red | 0.3 ng/band | Non-toxic, red fluorescent staining | 310 nm, 540 nm UV- and blue light |
| ROTI®Methylene blue staining concentrate | 10 ng/band | Reversible, non-toxic blue staining | White light |
| ROTI®Black N | <0.1 ng/band | Silver staining of DNA polyacrylic amide gels | White light |
Certificates of Analysis
Type analysis
| Appearance | red, cryst. powder |
| Assay | ≥98.0 % |
| Solubility (in H2O) | clear, red |
| pH value (2 % in H2O) | 4.0-7.0 |
| Loss on drying | ≤10.0 % |