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Transfer membrane ROTI®NC 0.2, 300 × 30 cm

From  Carl ROTH
Material: nitrocellulose (100 %).
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Transfer membrane for protein analyses, Southern- and Northern-Blots.

High-quality transfer membrane for all detection systems - extremely suitable for all protein transfer and detection methods. The membrane stands out for its particularly high mechanical stability. The pure 100 % nitrocellulose quality membrane is easy to block and allows for background-free blots with perfect bands.
Product details

€405.30/Pack Qty. 

excl. VAT. | 1 roll(s) per Pack Qty.

Art. No. 1YXN.1

In stock
Delivery fast, simple and reliable!
from 6 Pack Qty. €385.04/Pack Qty.
from 24 Pack Qty. €364.77/Pack Qty.

Product details

Material: nitrocellulose (100 %).

Transfer membrane for protein analyses, Southern- and Northern-Blots.

High-quality transfer membrane for all detection systems - extremely suitable for all protein transfer and detection methods. The membrane stands out for its particularly high mechanical stability. The pure 100 % nitrocellulose quality membrane is easy to block and allows for background-free blots with perfect bands.



Transfer membrane ROTI®NC 0.2 

The pore size of 0,2 μm is suitable for proteins and peptides of all sizes. The best retention rate is achieved when blotting proteins of up to 70 kDa. Its excellent protein binding capacity not only efficiently prevents the blot from going through during transfer, but also guarantees highly sensitive detection of even low protein amounts.

A slight optical difference between the two membrane sides results from the membrane production and has no influence on the binding capacity or the blot quality of the two sides. Both sides can be used equally for transfer and detection.We recommend to always use the optically same side as “detection side” to achieve maximum reproducibility.

Optimised for proteins of ≤70 kDa. Western- and far-western-blot, colony- and plaque-lifting of nucleic acids and proteins, immunoblots, dot-blots.
Detection via radio activity, chemoluminescence colour.

Suitable for:
  • Western Blotting
  • Protein and Immunoblotting
  • Northern Blotting
  • Southern Blotting
  • Dot / Slot Blotting


Not a medical device / Not an IVD product
Technical Information
Dimensions 300 × 30 cm 
Version roll 
Pore size 0,2 µm
Bond hydrophobic 
Binding capacity 100 µg/cm²
Thickn. 150±50 µm
Immobilisation UV, baking (max. 80 °C) 
Transfer membrane ROTI®NC 0.2
Selected quantity:   0
  1. Subtotal:  0.00
Art. No. Dimensions Pack Qty. Price Quantity
1YXN.1 300 × 30 cm 1 roll(s)

€405.30

200E.1 300 × 20 cm 1 roll(s)

€277.35

200H.1 20 × 20 cm 5 sheet(s)

€135.65

204H.1 10 × 10 cm 10 sheet(s)

€124.25

In stock
Available
In procurement
No longer available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS

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General information

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Roti® PVDF 0.2 Polyvinyliden fluoride hydrophobic 140 ±20 ≥125 0,2 not necessary
Roti® PVDF Polyvinyliden fluoride hydrophobic 125 ±25 ca. 200 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Roti® NC Nitrocellulose electrostatic, hydrophobic 150 ±50 125-200 0,2 UV, baking (max. 80 °C)
Roti® NC 0.45 Nitrocellulose electrostatic, hydrophobic 150 ±10 ≥125 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not