Technical Data Sheet
ROTI®Blue, 1 l
Ready-to-use products and reagents
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 8 III
WGK 1
UN-Nr. 1805
Colloidal Coomassie® staining solution for protein gels.
€117.20/Pack Qty.
excl. VAT. | 1 l per Pack Qty.
Art. No. A152.1
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Product details
ROTI®Blue 5x conc.
ROTI®Blue is a colloidal Coomassie® brilliant blue G250 staining agent for proteins in polyacrylamid gels. Its colloidal features enable the staining agent to be bound to the proteins with high specificity and only minimally to the gel matrix. There is no longer any need for the extensive washing steps for background decoloring.
- Most simple application
- No destaining necessary
- 5x more sensitive than Coomassie® R 250
A: Horizontal IEF for determination of wheat-varieties.
With kind permission of R. Knoblauch, LTZ Referat 23, Karlsruhe
B: SDS-PAGE ROTI®Mark Standard.
ROTI®Blue is approximately 5 x more sensitive than standard Coomassie® staining solutions. Staining time takes 2 to 12 hours depending on the desired band intensity.
Due to the fact that proteins may be stained without fixing the gel, membrane transfer and gel elution are still possible following staining.
Particularly well suited for staining prior to MS-analyses (e. g. MALDI) and prior to gel elution.
| Sensitivity | 10 |
| Use | Coomassie® staining solution for colloidal staining of proteins. Destaining not required. Non-fixing staining for protein gel elution possible. Proteomics-compatible. |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| A152.1 | 1 l | plastic |
€117.20 |
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| A152.2 | 250 ml | plastic |
€40.35 |
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In stock
Available
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
| Product | Sensitivity | Rapid Gel Analysis |
Gel Drying |
Elution/ Transfer |
2D- Gels |
MS- Analysis |
| ROTI®Black P | 1 ng/mm2 | - | +++ | - | +++ | - |
| ROTI®Blue | 10 ng/mm2 | - | +++ | +++ | - | +++ |
| ROTI®Blue quick | 10 ng/mm2 | +++ | +++ | ++ | - | ++ |
| ROTI®Blau R | 50 ng/mm2 | +++ | + | - | - | - |
| +++: recommended +: suitable -: not recommended | ||||||
Summary Staining of Protein Gels
| Product | Sensitivity | Description |
| ROTI®Black P | 1 ng/mm2 | Silver staining kit. Highly sensitive. Simple, fast and reproducible. |
| ROTI®Blue | 10 ng/mm2 | Coomassie®-solution for colloidal staining of proteins. Destaining not required. Non-fixing staining for protein gel elution possible. Proteomics-compatible. |
| ROTI®Blue quick | 10 ng/mm2 | Solution for rapid, highly specific staining of proteins. Destaining not required. Non-fixing staining, compatible with blotting and Proteomics. |
| ROTIPHORESE®Blau R | 50 ng/mm2 | Coomassie® staining solution concentrate for rapid staining. |
| Coomassie® Blue G250 (C.I. 42655) | 60 ng/mm2 | Dye for performing Coomassie® staining. ODmax = 595 |
| Coomassie® Blue R250 (C.I. 42660) | 50 ng/mm2 | Dye for performing Coomassie staining. ODmax = 550 |
Certificates of Analysis
Type analysis
| Appearance | dark blue, colloidale solution |
| pH-value | 1 - 4 |