Technical Data Sheet
ROTI®GelStain Red Eco, 5 ml, 5 x 1 ml
Products with optimised manufacturing process in terms of sustainability.
Ready-to-use products and reagents
Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
1 ml is sufficient for staining approx. 300 mini-gels with 30 ml agarose volume each.
€589.10/Pack Qty. Campaign price!
excl. VAT. | 5 ml per Pack Qty.
Art. No. 223C.2
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Product details
Non-toxic and non-carcinogenic dyes for fluorescent staining of nucleic acids in agarose gels and polyacrylamide gels. Highly sensitive and applied just like ethidium bromide.
The ROTI®GelStain dyes are used as additives to the gel solution and the running buffer, partly also post-run staining is possible. Both ROTI®GelStain dyes can be excited by UV light and by blue light. Resulting signals may be detected by ethidium bromide broadband photo filters.
ROTI®GelStain Red Eco 10 000x conc., ready-to-use
ROTI®GelStain Red Eco is a versatile staining reagent for the detection of all nucleic acids in agarose and polyacrylamide gels.
- Red fluorescence
- Alternative to ethidium bromide, non-toxic, non-mutagenic
- For staining of dsDNA, ssDNA and RNA
- Twice as sensitive as ethidium bromide (approx. 0.1 ng /band)
- Can be used with the common ethidium bromide filters
- Excitation with UV light (300 nm) and blue light (500 nm)
- Compatible with all common downstream applications
ROTI®GelStain Red Eco is a red fluorescent dye which, like ethidium bromide, can be used to stain nucleic acids. For this purpose, the dye can either be added directly to the gel solution or used for post-run staining.
With the Ames test, it could be proven that ROTI®GelStain Red Ecois significantly less toxic than ethidium bromide. At the same time, the dye is twice as sensitive as ethidium bromide (approx. 0.1 ng/band). The red fluorescence of the dye can be documented with all common ethidium bromide photofilters.
ROTI®GelStain Red Eco is compatible with all common downstream applications.
- More sensitive than ethidium bromide and ROTI®GelStain Red (0984.1)
- Dissolved in water instead of DMSO
- Also suitable for post-run staining
Excitation maximum(bound to DNA): approx. 300 nm and 500 nm
Emission maximum (bound to DNA): 600 nm.
| Max. excitation (DNA-bound) |
300 nm (UV) nm 500 nm (LED) nm |
| Emissions max. (DNA-bound) | 600 nm |
| Colour of the solution | red |
| Additional info | Store in the dark at +15 to +25°C. Do not freeze, do not cool! Short exposure (few days) to +4°C temperature is not critical, but may cause precipitation which disappears after warming up. |
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| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 223C.1 | 1 ml | plastic | 1 x 1 ml |
€155.90
€124.70 |
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| 223C.2 | 5 ml | plastic | 5 x 1 ml |
€736.40
€589.10 |
|
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In stock
Available
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
Summary Staining of Nucleic Acid in Gels
| Product | Sensitivity | Description | Excitation |
| Ethidium bromide solution | 0.5-5 ng/band | Standard fluorescent staining | 254-330 nm, 500 nm UV- and blue light |
| SYBR® Green DNA dye | 0.01 ng/band | Non-toxic, green fluorescent staining | 254 nm and 495 nm UV- and blue light |
| ROTI®GelStain | 0.3 ng/band | Non-toxic, green fluorescent staining | 302 nm and 490 nm UV- and blue light |
| ROTI®GelStain Red | 0.3 ng/band | Non-toxic, red fluorescent staining | 310 nm, 540 nm UV- and blue light |
| ROTI®Methylene blue staining concentrate | 10 ng/band | Reversible, non-toxic blue staining | White light |
| ROTI®Black N | <0.1 ng/band | Silver staining of DNA polyacrylic amide gels | White light |
Type analysis
| Appearance | red, transparent liquid, no precipitation |
| Staining of DNA in agarose gels | complies |
| Staining of DNA in PA gels | complies |
| DNase, RNase | not detectable |